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2,601 to 2,610 of 2,669 Results
FlowVis(Universität Stuttgart)
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Feb 4, 2020Visualisierungsinstitut der Universität Stuttgart
Deep enzymology data related to Gao et al.: Comprehensive structure-function characterization of DNMT3B and DNMT3A reveals distinctive de novo DNA methylation mechanisms
Deep enzymology data related to Gao et al.: Comprehensive structure-function characterization of DNMT3B and DNMT3A reveals distinctive de novo DNA methylation mechanisms
Jan 30, 2020 - Amplicon based bisulfite NGS data
Jeltsch, Albert; Bashtrykov, Pavel; Emperle, Max; Adam, Sabrina; Dukatz, Michael, 2020, "Deep enzymology data related to Gao et al.: Comprehensive structure-function characterization of DNMT3B and DNMT3A reveals distinctive de novo DNA methylation mechanisms", https://doi.org/10.18419/DARUS-627, DaRUS, V1
Experimental procedures: Libraries of double stranded DNA substrates with CpG, CpH or CpN sites in randomized sequence context were methlyated by DNMT3A or DNMT3B. Reactions were stopped by shock freezing in liquid nitrogen, then treated with proteinase K for 2 hours. Afterwards, the DNA was digested with the BsaI-HFv2 enzyme and a hairpin was liga...
Deep enzymology data related to Adam et al.: DNA sequence-dependent activity and base flipping mechanisms of DNMT1 regulate genome-wide DNA methylation
Deep enzymology data related to Adam et al.: DNA sequence-dependent activity and base flipping mechanisms of DNMT1 regulate genome-wide DNA methylation
Jan 30, 2020 - Amplicon based bisulfite NGS data
Jeltsch, Albert; Bashtrykov, Pavel; Adam, Sabrina, 2020, "Deep enzymology data related to Adam et al.: DNA sequence-dependent activity and base flipping mechanisms of DNMT1 regulate genome-wide DNA methylation", https://doi.org/10.18419/DARUS-629, DaRUS, V1
Methylation of long hemimethylated DNA substrates: Methylation of the 349 bp long hemimethylated substrate with DNMT1 was carried out in 1X methylation buffer (100 mM HEPES, 1 mM EDTA, 0.5 mM DTT, 0.1 mg/mL BSA, pH 7.2 with KOH) in the presence of 1 mM AdoMet. For the methylation reactions, mixtures containing different DNMT1 concentrations were pr...
Deep enzymology data related to Adam et al.: DNA sequence-dependent activity and base flipping mechanisms of DNMT1 regulate genome-wide DNA methylation
Deep enzymology data related to Adam et al.: DNA sequence-dependent activity and base flipping mechanisms of DNMT1 regulate genome-wide DNA methylation
Jan 30, 2020 - Amplicon based bisulfite NGS data
Jeltsch, Albert; Bashtrykov, Pavel; Adam, Sabrina, 2020, "Deep enzymology data related to Adam et al.: DNA sequence-dependent activity and base flipping mechanisms of DNMT1 regulate genome-wide DNA methylation", https://doi.org/10.18419/DARUS-628, DaRUS, V1
Methylation of random flank substrates: Libraries of double stranded DNA substrates with unmethylated or hemimethylated CpG sites in randomized sequence context were methlyated by DNMT1. Reactions were stopped by shock freezing in liquid nitrogen, then treated with proteinase K for 2 hours. Afterwards, the DNA was digested with the BsaI-HFv2 enzyme...
GraphML files for protein sequence networks of expansin homologues
Jan 30, 2020 - Expansin Engineering Database
Lohoff, Caroline, 2020, "GraphML files for protein sequence networks of expansin homologues", https://doi.org/10.18419/DARUS-624, DaRUS, V1
GraphML files for undirected weighted graphs with nodes that represent protein sequences of expansin homologues. Protein sequences were clustered by a threshold of sequence identity to derive representative sequences.Pairwise sequence identity between two sequences was derived from global Needleman-Wunsch alignment. Protein sequence networks were g...
Profile hidden Markov models of the ExED
Jan 30, 2020 - Expansin Engineering Database
Lohoff, Caroline, 2020, "Profile hidden Markov models of the ExED", https://doi.org/10.18419/DARUS-623, DaRUS, V1
Profile hidden Markov models and their underlying multiple sequence alignments for the N- and C-terminal protein domains of expansins.
Query sequences for the update of the ExED
Jan 30, 2020 - Expansin Engineering Database
Lohoff, Caroline; Buchholz, Patrick C. F., 2020, "Query sequences for the update of the ExED", https://doi.org/10.18419/DARUS-622, DaRUS, V1, UNF:6:eFcDsa8udm3F11lB7M2q6w== [fileUNF]
Query sequences for the individual BLAST searches used to update the Expansin Engineering Database (ExED, https://exed.biocatnet.de/).
Amplicon based bisulfite NGS data(Universität Stuttgart)
Jan 29, 2020Department of Molecular Biochemistry
Department of Molecular Biochemistry(Universität Stuttgart)
Jan 29, 2020Institute of Biochemistry
Benchmark C1.2 - Numerical results reference solution
Jan 28, 2020 - RootWaterUptake
Koch, Timo, 2020, "Benchmark C1.2 - Numerical results reference solution", https://doi.org/10.18419/DARUS-471, DaRUS, V1
Root water uptake simulation with a numerical scheme where the root-soil surface is explicitly resolved by the computational grid. The roots are a one-dimensional network grid embedded in a 3D soil domain. This dataset contains the grid files for root and soil. 8-day-old lupine, segmented from MRI measurements (courtesy of Magdalena Landl, FZ Jülic...
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