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Jan 30, 2020 - Amplicon based bisulfite NGS data
Jeltsch, Albert; Bashtrykov, Pavel; Adam, Sabrina, 2020, "Deep enzymology data related to Adam et al.: DNA sequence-dependent activity and base flipping mechanisms of DNMT1 regulate genome-wide DNA methylation", https://doi.org/10.18419/DARUS-628, DaRUS, V1
Methylation of random flank substrates: Libraries of double stranded DNA substrates with unmethylated or hemimethylated CpG sites in randomized sequence context were methlyated by DNMT1. Reactions were stopped by shock freezing in liquid nitrogen, then treated with proteinase K for 2 hours. Afterwards, the DNA was digested with the BsaI-HFv2 enzyme...
Tabular Data - 8.1 KB - 1 Variables, 510 Observations - UNF:6:9nlqS8HIWJ5ngSIjGG58OQ==
Tabular file with headers: CBM63 with both domains (1st column), CBM63 with N-terminal domain (2nd column), CBM63 with C-terminal domain (3rd column). The expansin domains were annotated using a minimal domain-based score of 35.
Jan 30, 2020 - Expansin Engineering Database
Lohoff, Caroline, 2020, "GraphML files for protein sequence networks of expansin homologues", https://doi.org/10.18419/DARUS-624, DaRUS, V1
GraphML files for undirected weighted graphs with nodes that represent protein sequences of expansin homologues. Protein sequences were clustered by a threshold of sequence identity to derive representative sequences.Pairwise sequence identity between two sequences was derived from global Needleman-Wunsch alignment. Protein sequence networks were g...
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