Mammalian Cell Culture

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Optimum Blue Light Exposure: A Means to Increase Cell-Specific Productivity in Chinese Hamster Ovary Cells Nov 29, 2024 Föller, Stefanie; Takors, Ralf; Regett, Niklas; Levin Lataster; Radziwill, Gerald, 2024, "Optimum Blue Light Exposure: A Means to Increase Cell-Specific Productivity in Chinese Hamster Ovary Cells", https://doi.org/10.18419/DARUS-4449, DaRUS, V1 Raw Data for manuscript titled Optimum Blue Light Exposure: A Means to Increase Cell-Specific Productivity in Chinese Hamster Ovary Cells This dataset was generated from experiments conducted in mini bioreactor cultures to investigate the effects of blue light illumination on mammalian cells. The study demonstrated an increase in cell-specific prod...
Figure 1.csv Nov 29, 2024 - Optimum Blue Light Exposure: A Means to Increase Cell-Specific Productivity in Chinese Hamster Ovary Cells Comma Separated Values - 2.9 KB - MD5: 762866f9d4837df512eb8b88c1e06f20 CSV file for data for figure 1: CHO DP-12 illuminated by blue LED light and treated with reduced glutathione. Viable cell density measured via holographic microscopy, normalized to the starting point, representing x-fold changes. Calculated specific growth rate over the course of the whole cultivation in 1*d-1. Cell-specific productivity (csp) calc...
Figure 1.xlsx Nov 29, 2024 - Optimum Blue Light Exposure: A Means to Increase Cell-Specific Productivity in Chinese Hamster Ovary Cells MS Excel Spreadsheet - 13.3 KB - MD5: 5e9a50e596b36293fa3a950aefef5e53 data for figure 1: CHO DP-12 illuminated by blue LED light and treated with reduced glutathione. Viable cell density measured via holographic microscopy, normalized to the starting point, representing x-fold changes. Calculated specific growth rate over the course of the whole cultivation in 1*d-1. Cell-specific productivity (csp) calculated via th...
Figure 2.csv Nov 29, 2024 - Optimum Blue Light Exposure: A Means to Increase Cell-Specific Productivity in Chinese Hamster Ovary Cells Comma Separated Values - 3.5 KB - MD5: 6a5611c6c398f08f5ab4d45dc5a8da6a CSV file for figure 2: CHO DP-12 illuminated by blue LED light with different illumination intensities and intervals. Viable cell density measure via holographic microscopy, normalized to the starting point, equaling x-fold changes. Calculated specific growth rate over the course of the whole cultivation in 1d-1. Specific growth rate in 1d-1 over...
Figure 2.xlsx Nov 29, 2024 - Optimum Blue Light Exposure: A Means to Increase Cell-Specific Productivity in Chinese Hamster Ovary Cells MS Excel Spreadsheet - 16.6 KB - MD5: 1c979f1ffd3985fba5a316a2049217e8 Data for figure 2: CHO DP-12 illuminated by blue LED light with different illumination intensities and intervals. Viable cell density measure via holographic microscopy, normalized to the starting point, equaling x-fold changes. Calculated specific growth rate over the course of the whole cultivation in 1d-1. Specific growth rate in 1d-1 over the...
Figure 3.csv Nov 29, 2024 - Optimum Blue Light Exposure: A Means to Increase Cell-Specific Productivity in Chinese Hamster Ovary Cells Comma Separated Values - 1.0 KB - MD5: e3ca3f64c1f3fd49d72e035df169933e CSV file for figure 3: Cell-specific productivity (csp) between 48-96 h. Cell-specific productivity calculated via the antibody titer detected by enzyme-linked immunosorbent assay and subsequently divided by the integral viable cell concentration in the timeframe of 48-96 h and normalized to the control. Longest lighting interval 10 min illuminatio...
Figure 3.xlsx Nov 29, 2024 - Optimum Blue Light Exposure: A Means to Increase Cell-Specific Productivity in Chinese Hamster Ovary Cells MS Excel Spreadsheet - 9.6 KB - MD5: a68cafcbbefc3beb12a98b90750e0dc5 Data for figure 3: Cell-specific productivity (csp) between 48-96 h. Cell-specific productivity calculated via the antibody titer detected by enzyme-linked immunosorbent assay and subsequently divided by the integral viable cell concentration in the timeframe of 48-96 h and normalized to the control. Longest lighting interval 10 min illumination/20...
Figure 4.csv Nov 29, 2024 - Optimum Blue Light Exposure: A Means to Increase Cell-Specific Productivity in Chinese Hamster Ovary Cells Comma Separated Values - 1.7 KB - MD5: 454ae9e245e0e6150d9840c4233d263c CSV file for figure 4: Cell cycle phase distribution of CHO DP-12 cells. Percentage of cells in G1-phase, S-phase and G2/M-phase for cells kept in the dark, cells illuminated with 12 W/m² for 10 min intervals (12 W/m² 10min), and cells illuminated permanently with 0.25 W/m² . G1-phase distribution compared to a positive control cells treated with 2...
Figure 4.xlsx Nov 29, 2024 - Optimum Blue Light Exposure: A Means to Increase Cell-Specific Productivity in Chinese Hamster Ovary Cells MS Excel Spreadsheet - 10.5 KB - MD5: 1298bdcb060e1a96396a22269f6258cb data for figure 4: Cell cycle phase distribution of CHO DP-12 cells. Percentage of cells in G1-phase, S-phase and G2/M-phase for cells kept in the dark, cells illuminated with 12 W/m² for 10 min intervals (12 W/m² 10min), and cells illuminated permanently with 0.25 W/m² . G1-phase distribution compared to a positive control cells treated with 2% di...
QTOF Fingerprint hits.csv Nov 29, 2024 - Optimum Blue Light Exposure: A Means to Increase Cell-Specific Productivity in Chinese Hamster Ovary Cells Comma Separated Values - 12.8 KB - MD5: af788fc196fa7c7f6666f2f69adb8793 Supplementary: QTOF hits with > 1.1 fold change of analyzed medium illuminated with blue LED light (4 W*h/m²) compared to control medium kept in the dark. CSV file

Optimum Blue Light Exposure: A Means to Increase Cell-Specific Productivity in Chinese Hamster Ovary Cells

Nov 29, 2024

Föller, Stefanie; Takors, Ralf; Regett, Niklas; Levin Lataster; Radziwill, Gerald, 2024, "Optimum Blue Light Exposure: A Means to Increase Cell-Specific Productivity in Chinese Hamster Ovary Cells", https://doi.org/10.18419/DARUS-4449, DaRUS, V1

Raw Data for manuscript titled Optimum Blue Light Exposure: A Means to Increase Cell-Specific Productivity in Chinese Hamster Ovary Cells This dataset was generated from experiments conducted in mini bioreactor cultures to investigate the effects of blue light illumination on mammalian cells. The study demonstrated an increase in cell-specific prod...

Figure 1.csv

Nov 29, 2024 - Optimum Blue Light Exposure: A Means to Increase Cell-Specific Productivity in Chinese Hamster Ovary Cells

Comma Separated Values - 2.9 KB -

CSV file for data for figure 1: CHO DP-12 illuminated by blue LED light and treated with reduced glutathione. Viable cell density measured via holographic microscopy, normalized to the starting point, representing x-fold changes. Calculated specific growth rate over the course of the whole cultivation in 1*d-1. Cell-specific productivity (csp) calc...

Figure 1.xlsx

Nov 29, 2024 - Optimum Blue Light Exposure: A Means to Increase Cell-Specific Productivity in Chinese Hamster Ovary Cells

MS Excel Spreadsheet - 13.3 KB -

data for figure 1: CHO DP-12 illuminated by blue LED light and treated with reduced glutathione. Viable cell density measured via holographic microscopy, normalized to the starting point, representing x-fold changes. Calculated specific growth rate over the course of the whole cultivation in 1*d-1. Cell-specific productivity (csp) calculated via th...

Figure 2.csv

Nov 29, 2024 - Optimum Blue Light Exposure: A Means to Increase Cell-Specific Productivity in Chinese Hamster Ovary Cells

Comma Separated Values - 3.5 KB -

CSV file for figure 2: CHO DP-12 illuminated by blue LED light with different illumination intensities and intervals. Viable cell density measure via holographic microscopy, normalized to the starting point, equaling x-fold changes. Calculated specific growth rate over the course of the whole cultivation in 1*d-1. Specific growth rate in 1*d-1 over...

Figure 2.xlsx

Nov 29, 2024 - Optimum Blue Light Exposure: A Means to Increase Cell-Specific Productivity in Chinese Hamster Ovary Cells

MS Excel Spreadsheet - 16.6 KB -

Data for figure 2: CHO DP-12 illuminated by blue LED light with different illumination intensities and intervals. Viable cell density measure via holographic microscopy, normalized to the starting point, equaling x-fold changes. Calculated specific growth rate over the course of the whole cultivation in 1*d-1. Specific growth rate in 1*d-1 over the...

Figure 3.csv

Nov 29, 2024 - Optimum Blue Light Exposure: A Means to Increase Cell-Specific Productivity in Chinese Hamster Ovary Cells

Comma Separated Values - 1.0 KB -

CSV file for figure 3: Cell-specific productivity (csp) between 48-96 h. Cell-specific productivity calculated via the antibody titer detected by enzyme-linked immunosorbent assay and subsequently divided by the integral viable cell concentration in the timeframe of 48-96 h and normalized to the control. Longest lighting interval 10 min illuminatio...

Figure 3.xlsx

Nov 29, 2024 - Optimum Blue Light Exposure: A Means to Increase Cell-Specific Productivity in Chinese Hamster Ovary Cells

MS Excel Spreadsheet - 9.6 KB -

Data for figure 3: Cell-specific productivity (csp) between 48-96 h. Cell-specific productivity calculated via the antibody titer detected by enzyme-linked immunosorbent assay and subsequently divided by the integral viable cell concentration in the timeframe of 48-96 h and normalized to the control. Longest lighting interval 10 min illumination/20...

Figure 4.csv

Nov 29, 2024 - Optimum Blue Light Exposure: A Means to Increase Cell-Specific Productivity in Chinese Hamster Ovary Cells

Comma Separated Values - 1.7 KB -

CSV file for figure 4: Cell cycle phase distribution of CHO DP-12 cells. Percentage of cells in G1-phase, S-phase and G2/M-phase for cells kept in the dark, cells illuminated with 12 W/m² for 10 min intervals (12 W/m² 10min), and cells illuminated permanently with 0.25 W/m² . G1-phase distribution compared to a positive control cells treated with 2...

Figure 4.xlsx

Nov 29, 2024 - Optimum Blue Light Exposure: A Means to Increase Cell-Specific Productivity in Chinese Hamster Ovary Cells

MS Excel Spreadsheet - 10.5 KB -

data for figure 4: Cell cycle phase distribution of CHO DP-12 cells. Percentage of cells in G1-phase, S-phase and G2/M-phase for cells kept in the dark, cells illuminated with 12 W/m² for 10 min intervals (12 W/m² 10min), and cells illuminated permanently with 0.25 W/m² . G1-phase distribution compared to a positive control cells treated with 2% di...

QTOF Fingerprint hits.csv

Nov 29, 2024 - Optimum Blue Light Exposure: A Means to Increase Cell-Specific Productivity in Chinese Hamster Ovary Cells

Comma Separated Values - 12.8 KB -

Supplementary: QTOF hits with > 1.1 fold change of analyzed medium illuminated with blue LED light (4 W*h/m²) compared to control medium kept in the dark. CSV file

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