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MS Excel Spreadsheet - 20.0 KB -
MD5: 2aff5c336ac96f5ffe73265db2a3206e
Description how to use the raw sequencing data files provided in this repository. |
Adobe PDF - 88.9 KB -
MD5: 87197499fe59431b4dccba8501193e04
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Mar 4, 2024
Jeltsch, Albert; Bashtrykov, Pavel; Dossmann, Leonie; Emperle, Max, 2024, "NGS data related to Dossmann et al.: Specific DNMT3C flanking sequence preferences facilitate methylation of young murine retrotransposons", https://doi.org/10.18419/darus-3386, DaRUS, V1
Cloning and site-directed mutagenesis The gene of the catalytic, C-terminal domain of murine DNMT3C (amino acid residues 439-740 of P0DOY1) was obtained in E. coli codon optimized form from IDT Integrated DNA Technologies. The gene fragment was cloned with the StrataClone PCR Clo... |
Jan 10, 2024
Jeltsch, Albert; Bashtrykov, Pavel; Albrecht, Claudia, 2024, "NGS data related to Albrecht et al.: Locus specific and stable DNA demethylation at the H19/IGF2 ICR1 by epigenome editing using a dCas9-SunTag system and the catalytic domain of TET1", https://doi.org/10.18419/darus-3790, DaRUS, V1
Method overview For targeted DNA demethylation of the H19/IGF2 ICR1, HEK293 cells were transfected with two plasmids, one containing dCas9 fused to a SunTag with five repeats of the GCN4 peptide, separated by 22 aa long linkers, and scFv-fused TET1CD, as well as a GFP reporter pr... |
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MD5: 3658de0085cae3ca8cb2fc4352d0137a
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MD5: b491e133998e0af6394a0a34c36b1abd
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MD5: b18fcbd3ca9b75707ce0da0fd1f9d6df
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MD5: 73e0d4bb5cfe4d8e2d4d55f9f18094a4
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MD5: 566adf593d9dc3c110af9804a38c67e5
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Unknown - 10.9 MB -
MD5: 5d4a36bca752cc6c9f840133bd8be60c
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