Amplicon based bisulfite NGS data

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1 to 4 of 4 Results

Deep enzymology data related to Dukatz et al.: Complex DNA sequence readout mechanisms of the DNMT3B DNA methyltransferase. Jun 22, 2020 Jeltsch, Albert, 2020, "Deep enzymology data related to Dukatz et al.: Complex DNA sequence readout mechanisms of the DNMT3B DNA methyltransferase.", https://doi.org/10.18419/darus-815, DaRUS, V2 Experimental procedures: Libraries of double stranded DNA substrates with CpG, CpH or CpN sites in randomized sequence context were methlyated by DNMT3B. Reactions were stopped by shock freezing in liquid nitrogen, then treated with proteinase K for 2 hours. Afterwards, the DNA w...
Deep enzymology data related to Gao et al.: Comprehensive structure-function characterization of DNMT3B and DNMT3A reveals distinctive de novo DNA methylation mechanisms. Jan 30, 2020 Jeltsch, Albert, 2020, "Deep enzymology data related to Gao et al.: Comprehensive structure-function characterization of DNMT3B and DNMT3A reveals distinctive de novo DNA methylation mechanisms.", https://doi.org/10.18419/darus-627, DaRUS, V1 Experimental procedures: Libraries of double stranded DNA substrates with CpG, CpH or CpN sites in randomized sequence context were methlyated by DNMT3A or DNMT3B. Reactions were stopped by shock freezing in liquid nitrogen, then treated with proteinase K for 2 hours. Afterwards,...
Deep enzymology data related to Adam et al.: Sequence context-dependent base flipping regulates DNMT1-mediated genome-wide DNA methylation Jan 30, 2020 Jeltsch, Albert, 2020, "Deep enzymology data related to Adam et al.: Sequence context-dependent base flipping regulates DNMT1-mediated genome-wide DNA methylation", https://doi.org/10.18419/darus-629, DaRUS, V1 Methylation of long hemimethylated DNA substrates: Methylation of the 349 bp long hemimethylated substrate with DNMT1 was carried out in 1X methylation buffer (100 mM HEPES, 1 mM EDTA, 0.5 mM DTT, 0.1 mg/mL BSA, pH 7.2 with KOH) in the presence of 1 mM AdoMet. For the methylation...
Deep enzymology data related to Adam et al.: Sequence context-dependent base flipping regulates DNMT1-mediated genome-wide DNA methylation Jan 30, 2020 Jeltsch, Albert, 2020, "Deep enzymology data related to Adam et al.: Sequence context-dependent base flipping regulates DNMT1-mediated genome-wide DNA methylation", https://doi.org/10.18419/darus-628, DaRUS, V1 Methylation of random flank substrates: Libraries of double stranded DNA substrates with unmethylated or hemimethylated CpG sites in randomized sequence context were methlyated by DNMT1. Reactions were stopped by shock freezing in liquid nitrogen, then treated with proteinase K f...

Deep enzymology data related to Dukatz et al.: Complex DNA sequence readout mechanisms of the DNMT3B DNA methyltransferase.

Jun 22, 2020

Jeltsch, Albert, 2020, "Deep enzymology data related to Dukatz et al.: Complex DNA sequence readout mechanisms of the DNMT3B DNA methyltransferase.", https://doi.org/10.18419/darus-815, DaRUS, V2

Experimental procedures: Libraries of double stranded DNA substrates with CpG, CpH or CpN sites in randomized sequence context were methlyated by DNMT3B. Reactions were stopped by shock freezing in liquid nitrogen, then treated with proteinase K for 2 hours. Afterwards, the DNA w...

Deep enzymology data related to Gao et al.: Comprehensive structure-function characterization of DNMT3B and DNMT3A reveals distinctive de novo DNA methylation mechanisms.

Jan 30, 2020

Jeltsch, Albert, 2020, "Deep enzymology data related to Gao et al.: Comprehensive structure-function characterization of DNMT3B and DNMT3A reveals distinctive de novo DNA methylation mechanisms.", https://doi.org/10.18419/darus-627, DaRUS, V1

Experimental procedures: Libraries of double stranded DNA substrates with CpG, CpH or CpN sites in randomized sequence context were methlyated by DNMT3A or DNMT3B. Reactions were stopped by shock freezing in liquid nitrogen, then treated with proteinase K for 2 hours. Afterwards,...

Deep enzymology data related to Adam et al.: Sequence context-dependent base flipping regulates DNMT1-mediated genome-wide DNA methylation

Jan 30, 2020

Jeltsch, Albert, 2020, "Deep enzymology data related to Adam et al.: Sequence context-dependent base flipping regulates DNMT1-mediated genome-wide DNA methylation", https://doi.org/10.18419/darus-629, DaRUS, V1

Methylation of long hemimethylated DNA substrates: Methylation of the 349 bp long hemimethylated substrate with DNMT1 was carried out in 1X methylation buffer (100 mM HEPES, 1 mM EDTA, 0.5 mM DTT, 0.1 mg/mL BSA, pH 7.2 with KOH) in the presence of 1 mM AdoMet. For the methylation...

Deep enzymology data related to Adam et al.: Sequence context-dependent base flipping regulates DNMT1-mediated genome-wide DNA methylation

Jan 30, 2020

Methylation of random flank substrates: Libraries of double stranded DNA substrates with unmethylated or hemimethylated CpG sites in randomized sequence context were methlyated by DNMT1. Reactions were stopped by shock freezing in liquid nitrogen, then treated with proteinase K f...

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