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1 to 10 of 17 Results

Raw data for: The knowledge driven DBTL cycle provides mechanistic insights while optimising dopamine production in Escherichia coli Apr 16, 2025 Hägele, Lorena; Takors, Ralf, 2025, "Raw data for: The knowledge driven DBTL cycle provides mechanistic insights while optimising dopamine production in Escherichia coli", https://doi.org/10.18419/DARUS-4714, DaRUS, V1 Dopamine is a promising organic compound with several key applications in emergency medicine, diagnosis and treatment of cancer, production of lithium anodes, and wastewater treatment. Since studies on in vivo dopamine production are limited, this study demonstrates the development and optimisation of a dopamine production strain by the help of the...
01_Host_strain_engineering.xlsx Apr 16, 2025 - Raw data for: The knowledge driven DBTL cycle provides mechanistic insights while optimising dopamine production in Escherichia coli MS Excel Spreadsheet - 321.3 KB - MD5: 205fdf5c2a4fd032285dd9388bdaa13e RoboLector Data for engineered hosts strains for tyrosine production. Also HPLC raw data and evaluation is added.
02_in vitro investigation.xlsx Apr 16, 2025 - Raw data for: The knowledge driven DBTL cycle provides mechanistic insights while optimising dopamine production in Escherichia coli MS Excel Spreadsheet - 76.5 KB - MD5: a2c1a159d554a8979d1108565b116c2a Protokoll and results of the in vitro crude cell lysate investigation.
03_high throughput Design.xlsx Apr 16, 2025 - Raw data for: The knowledge driven DBTL cycle provides mechanistic insights while optimising dopamine production in Escherichia coli MS Excel Spreadsheet - 45.6 KB - MD5: 858112b8858e128cf31a79391885abe2 Extracted RBS sequences with related TIR and further design of the high throughput RBS engineering.
04_high throughput_strain construction_testing.xlsx Apr 16, 2025 - Raw data for: The knowledge driven DBTL cycle provides mechanistic insights while optimising dopamine production in Escherichia coli MS Excel Spreadsheet - 2.4 MB - MD5: ddae7084aeaa29a081049947d7c1c8a9 Fully characterisation and documentation of the strain construction and testing.
05_GC content evaluation.xlsx Apr 16, 2025 - Raw data for: The knowledge driven DBTL cycle provides mechanistic insights while optimising dopamine production in Escherichia coli MS Excel Spreadsheet - 71.5 KB - MD5: 04403fd22673c8bbc71325b97d08476c Evaulation of the GC content.
06_Proteomics_evaluation.xlsx Apr 16, 2025 - Raw data for: The knowledge driven DBTL cycle provides mechanistic insights while optimising dopamine production in Escherichia coli MS Excel Spreadsheet - 1.4 MB - MD5: c3236f37ece405323d4477874799aacd Raw data and evaluation of the proteomics data.
Getting the right clones in an automated manner: an alternative to sophisticated colony-picking robotics Aug 2, 2024 Hägele, Lorena; Takors, Ralf, 2024, "Getting the right clones in an automated manner: an alternative to sophisticated colony-picking robotics", https://doi.org/10.18419/DARUS-4355, DaRUS, V1 In recent years, the Design-Build-Test-Learn (DBTL) cycle has become a key concept in strain engineering. Modern biofoundries enable automated DBTL cycling using robotic devices. However, both highly automated facilities and semi-automated facilities encounter bottlenecks in clone selection and screening. While fully automated biofoundries can take...
C.glutamicum_investigation agar plates.xlsx Aug 2, 2024 - Getting the right clones in an automated manner: an alternative to sophisticated colony-picking robotics MS Excel Spreadsheet - 67.9 KB - MD5: e79133871ed8ae10573b3f7e78f3995c
C.gultamicum_ALCS data.xlsx Aug 2, 2024 - Getting the right clones in an automated manner: an alternative to sophisticated colony-picking robotics MS Excel Spreadsheet - 4.8 MB - MD5: 14a90257f040871b6afb53e37ee34907

Raw data for: The knowledge driven DBTL cycle provides mechanistic insights while optimising dopamine production in Escherichia coli

Apr 16, 2025

Hägele, Lorena; Takors, Ralf, 2025, "Raw data for: The knowledge driven DBTL cycle provides mechanistic insights while optimising dopamine production in Escherichia coli", https://doi.org/10.18419/DARUS-4714, DaRUS, V1

Dopamine is a promising organic compound with several key applications in emergency medicine, diagnosis and treatment of cancer, production of lithium anodes, and wastewater treatment. Since studies on in vivo dopamine production are limited, this study demonstrates the development and optimisation of a dopamine production strain by the help of the...

01_Host_strain_engineering.xlsx

Apr 16, 2025 - Raw data for: The knowledge driven DBTL cycle provides mechanistic insights while optimising dopamine production in Escherichia coli

MS Excel Spreadsheet - 321.3 KB -

RoboLector Data for engineered hosts strains for tyrosine production. Also HPLC raw data and evaluation is added.

02_in vitro investigation.xlsx

Apr 16, 2025 - Raw data for: The knowledge driven DBTL cycle provides mechanistic insights while optimising dopamine production in Escherichia coli

MS Excel Spreadsheet - 76.5 KB -

Protokoll and results of the in vitro crude cell lysate investigation.

03_high throughput Design.xlsx

Apr 16, 2025 - Raw data for: The knowledge driven DBTL cycle provides mechanistic insights while optimising dopamine production in Escherichia coli

MS Excel Spreadsheet - 45.6 KB -

Extracted RBS sequences with related TIR and further design of the high throughput RBS engineering.

04_high throughput_strain construction_testing.xlsx

Apr 16, 2025 - Raw data for: The knowledge driven DBTL cycle provides mechanistic insights while optimising dopamine production in Escherichia coli

MS Excel Spreadsheet - 2.4 MB -

Fully characterisation and documentation of the strain construction and testing.

05_GC content evaluation.xlsx

Apr 16, 2025 - Raw data for: The knowledge driven DBTL cycle provides mechanistic insights while optimising dopamine production in Escherichia coli

MS Excel Spreadsheet - 71.5 KB -

Evaulation of the GC content.

06_Proteomics_evaluation.xlsx

Apr 16, 2025 - Raw data for: The knowledge driven DBTL cycle provides mechanistic insights while optimising dopamine production in Escherichia coli

MS Excel Spreadsheet - 1.4 MB -

Raw data and evaluation of the proteomics data.

Getting the right clones in an automated manner: an alternative to sophisticated colony-picking robotics

Aug 2, 2024

Hägele, Lorena; Takors, Ralf, 2024, "Getting the right clones in an automated manner: an alternative to sophisticated colony-picking robotics", https://doi.org/10.18419/DARUS-4355, DaRUS, V1

In recent years, the Design-Build-Test-Learn (DBTL) cycle has become a key concept in strain engineering. Modern biofoundries enable automated DBTL cycling using robotic devices. However, both highly automated facilities and semi-automated facilities encounter bottlenecks in clone selection and screening. While fully automated biofoundries can take...

C.glutamicum_investigation agar plates.xlsx

Aug 2, 2024 - Getting the right clones in an automated manner: an alternative to sophisticated colony-picking robotics

MS Excel Spreadsheet - 67.9 KB -

C.gultamicum_ALCS data.xlsx

Aug 2, 2024 - Getting the right clones in an automated manner: an alternative to sophisticated colony-picking robotics

MS Excel Spreadsheet - 4.8 MB -

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