Bioprocess

Featured Dataverses

In order to use this feature you must have at least one published or linked dataverse.

Publish Dataverse

Are you sure you want to publish your dataverse? Once you do so it must remain published.

Publish Dataverse

This dataverse cannot be published because the dataverse it is in has not been published.

Delete Dataverse

Are you sure you want to delete your dataverse? You cannot undelete this dataverse.

31 to 40 of 66 Results

Model Information_E_Coli_AMD_MicroAerobic_29102024.xls Nov 19, 2024 - Data for: Substitute yeast extract while maintaining performance: showcase amorpha-4,11-diene production MS Excel Spreadsheet - 451.0 KB - MD5: 0ba909529e39395096169cb7faf9b702
ReactionList.tab Nov 19, 2024 - Data for: Substitute yeast extract while maintaining performance: showcase amorpha-4,11-diene production Tabular Data - 236.9 KB - 9 Variables, 1074 Observations - UNF:6:YEF5Ajp8e2Cuk3iyclzs6w==
Assessing nucleotide sugar donors inside the Golgi apparatus as a prerequisite for unravelling culture impacts on glycoforms of antibodies Oct 28, 2024 Regett, Niklas; Marcel Dieterle; Fleur Peters; Max Deuring; Kaja Stegmaier; Teleki, Attila; Takors, Ralf, 2024, "Assessing nucleotide sugar donors inside the Golgi apparatus as a prerequisite for unravelling culture impacts on glycoforms of antibodies", https://doi.org/10.18419/DARUS-4541, DaRUS, V1, UNF:6:sZ6QKMB2+sxpW+LZIhLK2Q== [fileUNF] The dataset presented here supplements the publication titled “Assessing Nucleotide Sugar Donors Inside the Golgi Apparatus as a Prerequisite for Unraveling Culture Impacts on Glycoforms of Antibodies.” The study emphasizes the importance of glycosylation in biopharmaceuticals and unravels a novel, integrative workflow for absolute quantification o...
Figure1.xlsx Oct 28, 2024 - Assessing nucleotide sugar donors inside the Golgi apparatus as a prerequisite for unravelling culture impacts on glycoforms of antibodies MS Excel Spreadsheet - 10.2 KB - MD5: f07c5e2560524fa3b8ddb4f201f24100 Averaged protein mass in subcellular protein fractions. Protein mass [mg] over increasing digitonin concentration [µg/mL] in cytoplasm fractionation buffer. Fractions are cytoplasm, organelles, or the remainder after fractionation.
Figure4.xlsx Oct 28, 2024 - Assessing nucleotide sugar donors inside the Golgi apparatus as a prerequisite for unravelling culture impacts on glycoforms of antibodies MS Excel Spreadsheet - 12.3 KB - MD5: 2d1420f554fea00d192bbba09b71d18f Averaged metabolite concentration in subcellular metabolite fractions. Cellular metabolite concentration over increasing digitonin concentration in cytoplasm fractionation buffer. Fractions are cytoplasm and, organelles.
Figure5.tab Oct 28, 2024 - Assessing nucleotide sugar donors inside the Golgi apparatus as a prerequisite for unravelling culture impacts on glycoforms of antibodies Tabular Data - 797 B - 4 Variables, 24 Observations - UNF:6:1v2ueH6QrpFch0T6vgGLbg== Data for the sigmoid fit to metabolite concentrations. Averaged and normalized analyte concentrations from organelle- or cytoplasm fractions over increasing digitonin concentration in fractionation buffer.
Figure6.tab Oct 28, 2024 - Assessing nucleotide sugar donors inside the Golgi apparatus as a prerequisite for unravelling culture impacts on glycoforms of antibodies Tabular Data - 2.5 KB - 18 Variables, 20 Observations - UNF:6:mWkScrpAtXBjjTVXIe+lZQ== Averages of NSD concentrations in the cytoplasm and organelles. Measured over 6 days with different nutrient pulsing strategies applied on day 3: only medium (A); manganese, uridine, and fructose dissolved in medium (B); and manganese, uridine, and galactose dissolved in medium (C).
FigureS2.xlsx Oct 28, 2024 - Assessing nucleotide sugar donors inside the Golgi apparatus as a prerequisite for unravelling culture impacts on glycoforms of antibodies MS Excel Spreadsheet - 13.2 KB - MD5: 85439b0a6f08828c717f5c1b06669bb4 Averaged cultivation parameters of CHO shaking flask cultivations. Parameters under three different nutrient-pulsing regimes. Data show condition (A) with only medium spiked, fructose spiked medium with manganese and uridine (B), and galactose spiked medium with manganese and uridine (C).
FigureS3.tab Oct 28, 2024 - Assessing nucleotide sugar donors inside the Golgi apparatus as a prerequisite for unravelling culture impacts on glycoforms of antibodies Tabular Data - 1.3 KB - 14 Variables, 25 Observations - UNF:6:b19HAxpf2oV1MbnTDmcNfA== Averaged guanine oligophosphate levels. After concentration of fractions with lyophilization or evaporation.
FigureS4.tab Oct 28, 2024 - Assessing nucleotide sugar donors inside the Golgi apparatus as a prerequisite for unravelling culture impacts on glycoforms of antibodies Tabular Data - 566 B - 10 Variables, 8 Observations - UNF:6:9dNfmJTzfmiv55W/T8fLWg== Averaged NSD levels After concentration of fractions with lyophilization or evaporation.

Model Information_E_Coli_AMD_MicroAerobic_29102024.xls

Nov 19, 2024 - Data for: Substitute yeast extract while maintaining performance: showcase amorpha-4,11-diene production

MS Excel Spreadsheet - 451.0 KB -

ReactionList.tab

Nov 19, 2024 - Data for: Substitute yeast extract while maintaining performance: showcase amorpha-4,11-diene production

Tabular Data - 236.9 KB - 9 Variables, 1074 Observations -

Assessing nucleotide sugar donors inside the Golgi apparatus as a prerequisite for unravelling culture impacts on glycoforms of antibodies

Oct 28, 2024

Regett, Niklas; Marcel Dieterle; Fleur Peters; Max Deuring; Kaja Stegmaier; Teleki, Attila; Takors, Ralf, 2024, "Assessing nucleotide sugar donors inside the Golgi apparatus as a prerequisite for unravelling culture impacts on glycoforms of antibodies", https://doi.org/10.18419/DARUS-4541, DaRUS, V1, UNF:6:sZ6QKMB2+sxpW+LZIhLK2Q== [fileUNF]

The dataset presented here supplements the publication titled “Assessing Nucleotide Sugar Donors Inside the Golgi Apparatus as a Prerequisite for Unraveling Culture Impacts on Glycoforms of Antibodies.” The study emphasizes the importance of glycosylation in biopharmaceuticals and unravels a novel, integrative workflow for absolute quantification o...

Figure1.xlsx

Oct 28, 2024 - Assessing nucleotide sugar donors inside the Golgi apparatus as a prerequisite for unravelling culture impacts on glycoforms of antibodies

MS Excel Spreadsheet - 10.2 KB -

Averaged protein mass in subcellular protein fractions. Protein mass [mg] over increasing digitonin concentration [µg/mL] in cytoplasm fractionation buffer. Fractions are cytoplasm, organelles, or the remainder after fractionation.

Figure4.xlsx

Oct 28, 2024 - Assessing nucleotide sugar donors inside the Golgi apparatus as a prerequisite for unravelling culture impacts on glycoforms of antibodies

MS Excel Spreadsheet - 12.3 KB -

Averaged metabolite concentration in subcellular metabolite fractions. Cellular metabolite concentration over increasing digitonin concentration in cytoplasm fractionation buffer. Fractions are cytoplasm and, organelles.

Figure5.tab

Oct 28, 2024 - Assessing nucleotide sugar donors inside the Golgi apparatus as a prerequisite for unravelling culture impacts on glycoforms of antibodies

Tabular Data - 797 B - 4 Variables, 24 Observations -

Data for the sigmoid fit to metabolite concentrations. Averaged and normalized analyte concentrations from organelle- or cytoplasm fractions over increasing digitonin concentration in fractionation buffer.

Figure6.tab

Oct 28, 2024 - Assessing nucleotide sugar donors inside the Golgi apparatus as a prerequisite for unravelling culture impacts on glycoforms of antibodies

Tabular Data - 2.5 KB - 18 Variables, 20 Observations -

Averages of NSD concentrations in the cytoplasm and organelles. Measured over 6 days with different nutrient pulsing strategies applied on day 3: only medium (A); manganese, uridine, and fructose dissolved in medium (B); and manganese, uridine, and galactose dissolved in medium (C).

FigureS2.xlsx

Oct 28, 2024 - Assessing nucleotide sugar donors inside the Golgi apparatus as a prerequisite for unravelling culture impacts on glycoforms of antibodies

MS Excel Spreadsheet - 13.2 KB -

Averaged cultivation parameters of CHO shaking flask cultivations. Parameters under three different nutrient-pulsing regimes. Data show condition (A) with only medium spiked, fructose spiked medium with manganese and uridine (B), and galactose spiked medium with manganese and uridine (C).

FigureS3.tab

Oct 28, 2024 - Assessing nucleotide sugar donors inside the Golgi apparatus as a prerequisite for unravelling culture impacts on glycoforms of antibodies

Tabular Data - 1.3 KB - 14 Variables, 25 Observations -

Averaged guanine oligophosphate levels. After concentration of fractions with lyophilization or evaporation.

FigureS4.tab

Oct 28, 2024 - Assessing nucleotide sugar donors inside the Golgi apparatus as a prerequisite for unravelling culture impacts on glycoforms of antibodies

Tabular Data - 566 B - 10 Variables, 8 Observations -

Averaged NSD levels After concentration of fractions with lyophilization or evaporation.

Add Data

Share Dataverse

Link Dataverse

Reset Modifications