41 to 50 of 727 Results
Unknown - 8.9 MB -
MD5: b18fcbd3ca9b75707ce0da0fd1f9d6df
|
Unknown - 8.9 MB -
MD5: b491e133998e0af6394a0a34c36b1abd
|
Unknown - 5.9 MB -
MD5: 3658de0085cae3ca8cb2fc4352d0137a
|
Adobe PDF - 88.9 KB -
MD5: 87197499fe59431b4dccba8501193e04
|
Jan 12, 2024 - Amplicon based bisulfite NGS data
Jeltsch, Albert; Bashtrykov, Pavel; Rajaram, Nivethika, 2023, "NGS data related to Rajaram et al.: Development of super-specific epigenome editing by targeted allele-specific DNA methylation", https://doi.org/10.18419/darus-3581, DaRUS, V2
Method overview To achieve targeted ASM, transient transfection of the dCas9-10X SunTag-BFP, scFv-DNMT3A-3L-sfGFP, and sgRNA-DsRed plasmids was performed in HEK293 cells. Control experiments were conducted with a scrambled sgRNA that does not have a binding site in the human geno... |
Jan 12, 2024 -
NGS data related to Rajaram et al.: Development of super-specific epigenome editing by targeted allele-specific DNA methylation
MS Excel Spreadsheet - 29.7 KB -
MD5: d6ddba1c0d33a89731b57212cc659179
Information about connections of experiments and sequencing data |
Jan 10, 2024 - Amplicon based bisulfite NGS data
Jeltsch, Albert; Bashtrykov, Pavel; Albrecht, Claudia, 2024, "NGS data related to Albrecht et al.: Locus specific and stable DNA demethylation at the H19/IGF2 ICR1 by epigenome editing using a dCas9-SunTag system and the catalytic domain of TET1", https://doi.org/10.18419/darus-3790, DaRUS, V1
Method overview For targeted DNA demethylation of the H19/IGF2 ICR1, HEK293 cells were transfected with two plasmids, one containing dCas9 fused to a SunTag with five repeats of the GCN4 peptide, separated by 22 aa long linkers, and scFv-fused TET1CD, as well as a GFP reporter pr... |
Gzip Archive - 57.5 MB -
MD5: 5011f6791cb689554ae334baa1918394
|
Gzip Archive - 66.3 MB -
MD5: 179511fa9bda9093f74635aceafad658
|
Gzip Archive - 229.7 MB -
MD5: 51c57d1f4272c2fa4779b3c596366aca
|