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Jeltsch, Albert; Bashtrykov, Pavel, 2025, "NGS data related to Sogl et al. "Specificities and flanking sequence preferences of bacterial DNA-(cytosine C5)-methyltransferases"", https://doi.org/10.18419/DARUS-4515, DaRUS, V1, UNF:6:bSvQoJxvb+T/iyQGPOvTBA== [fileUNF]
Analysis of flanking sequence preference with a randomized substrate and bioinformatic data For analysis of the flanking sequence preference, substrate with different target sites sites in a 9 or 10 bp randomized sequence context were prepared as described (Dukatz, et al. 2020; Dukatz, et al. 2022). Substrate methylation reactions were performed wi... |
Jan 13, 2025 -
NGS data related to Sogl et al. "Specificities and flanking sequence preferences of bacterial DNA-(cytosine C5)-methyltransferases"
ZIP Archive - 177.1 MB -
MD5: 6e0120fb5866aaffd82db1fbfa76b252
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Jan 13, 2025 -
NGS data related to Sogl et al. "Specificities and flanking sequence preferences of bacterial DNA-(cytosine C5)-methyltransferases"
Adobe PDF - 104.6 KB -
MD5: 1fa8ecab0ba68b617138a98d90160ffc
Description of the data structure |
Jan 13, 2025 -
NGS data related to Sogl et al. "Specificities and flanking sequence preferences of bacterial DNA-(cytosine C5)-methyltransferases"
MS Excel Spreadsheet - 736.2 KB -
MD5: ddd9a622dbb5638621ca557e01fc4ec2
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Jan 13, 2025 -
NGS data related to Sogl et al. "Specificities and flanking sequence preferences of bacterial DNA-(cytosine C5)-methyltransferases"
Tabular Data - 53.3 KB - 8 Variables, 1106 Observations - UNF:6:bSvQoJxvb+T/iyQGPOvTBA==
Assignment of experiments and data files and overview of the number of reads in CSV format |
Jan 13, 2025 -
NGS data related to Sogl et al. "Specificities and flanking sequence preferences of bacterial DNA-(cytosine C5)-methyltransferases"
MS Excel Spreadsheet - 56.4 KB -
MD5: a3df4453d1eb9612fabd87027482cfbe
Assignment of experiments and data files and overview of the number of reads |
Aug 12, 2024
Jeltsch, Albert; Bashtrykov, Pavel; Rajaram, Nivethika, 2024, "NGS data related to Rajaram et al.: Allele specific DNA demethylation ...", https://doi.org/10.18419/DARUS-4230, DaRUS, V2
Method overview To achieve targeted locus and allele-specific DNA demethylation, HEK293 cells were transfected with two plasmids. One plasmid contains, dCas9 fused to a SunTag with five repeats of the GCN4 peptide, separated by 22 aa long linkers, and scFv-fused TET1CD, as well as a GFP reporter protein. The other plasmid is a multiguide plasmid wi... |
MS Excel Spreadsheet - 20.9 KB -
MD5: 1043e2ae35d805a68dced1d64ccf5772
Information about the uploaded sequencing files. |
Gzip Archive - 238.8 MB -
MD5: 05a29e71ba2585fed9b74305d8d72203
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Gzip Archive - 239.5 MB -
MD5: 968e236965a02405f40fdf91c34ce8ca
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