Institute of Biochemistry

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1,141 to 1,150 of 1,362 Results

Plastics-Active Enzymes Database(Universität Stuttgart) Nov 26, 2021Bioinformatics Supporting information and data for the PAZy database on plastics-active enzymes.
NGS data related to Bröhm et al.: Methylation of recombinant mononucleosomes by DNMT3A demonstrates efficient linker DNA methylation and a role of H3K36me3 Nov 25, 2021 - Amplicon based bisulfite NGS data Jeltsch, Albert; Bashtrykov, Pavel; Bröhm, Alexander; Dukatz, Michael; Adam, Sabrina, 2021, "NGS data related to Bröhm et al.: Methylation of recombinant mononucleosomes by DNMT3A demonstrates efficient linker DNA methylation and a role of H3K36me3", https://doi.org/10.18419/DARUS-1252, DaRUS, V2 Methylation experiments: For the competitive nucleosome methylation experiments, 0.6 pmol of each nucleosome variant were digested with MluI (NEB) for 60 min at 37°C in 10 µL NEB Cutsmart buffer (50 mM KOAc/20 mM Tris-acetate pH 7.9, 10 mM Magnesium Acetate, 100 µg/mL BSA) to remove residual unbound DNA. Afterwards, DNMT3A2 or DNMT3AC was added to...
DNMT3A2_Nuc_K4me3_R1x.fastqsanger Nov 25, 2021 - NGS data related to Bröhm et al.: Methylation of recombinant mononucleosomes by DNMT3A demonstrates efficient linker DNA methylation and a role of H3K36me3 Unknown - 104.0 MB - MD5: 907fe10336105defe50568be8204dc6f
DNMT3A2_Nuc_K4me3_R2x.fastqsanger Nov 25, 2021 - NGS data related to Bröhm et al.: Methylation of recombinant mononucleosomes by DNMT3A demonstrates efficient linker DNA methylation and a role of H3K36me3 Unknown - 28.3 MB - MD5: aea43ad76f4cf3409c16d42649977b7e
DNMT3A2_Nuc_K4me3_R3x.fastqsanger Nov 25, 2021 - NGS data related to Bröhm et al.: Methylation of recombinant mononucleosomes by DNMT3A demonstrates efficient linker DNA methylation and a role of H3K36me3 Unknown - 53.8 MB - MD5: e4087727e3dd84462581d467d682e5e2
DNMT3A2_Nuc_unmod_R1x.fastqsanger Nov 25, 2021 - NGS data related to Bröhm et al.: Methylation of recombinant mononucleosomes by DNMT3A demonstrates efficient linker DNA methylation and a role of H3K36me3 Unknown - 38.5 MB - MD5: 3dae0fa62689aab55c5f3c5823f8d76d
DNMT3A2_Nuc_unmod_R2x.fastqsanger Nov 25, 2021 - NGS data related to Bröhm et al.: Methylation of recombinant mononucleosomes by DNMT3A demonstrates efficient linker DNA methylation and a role of H3K36me3 Unknown - 5.3 MB - MD5: 59b244dbc216aca8631b909c87232024
DNMT3A2_Nuc_unmod_R3x.fastqsanger Nov 25, 2021 - NGS data related to Bröhm et al.: Methylation of recombinant mononucleosomes by DNMT3A demonstrates efficient linker DNA methylation and a role of H3K36me3 Unknown - 15.0 MB - MD5: 0c9f74c6b7a4c759094fe28bf030006a
DNMT3AC_DNMT3B3C_4mer_Nuc_unmod_R1x.fastqsanger Nov 25, 2021 - NGS data related to Bröhm et al.: Methylation of recombinant mononucleosomes by DNMT3A demonstrates efficient linker DNA methylation and a role of H3K36me3 Unknown - 28.6 MB - MD5: ec17dfec2f6f26740ee4c2614f739da8
DNMT3AC_DNMT3B3C_4mer_Nuc_unmod_R2x.fastqsanger Nov 25, 2021 - NGS data related to Bröhm et al.: Methylation of recombinant mononucleosomes by DNMT3A demonstrates efficient linker DNA methylation and a role of H3K36me3 Unknown - 170.6 MB - MD5: 6cd2e8edd928792227057e7c216f79a6

Plastics-Active Enzymes Database(Universität Stuttgart)

Nov 26, 2021Bioinformatics

Supporting information and data for the PAZy database on plastics-active enzymes.

NGS data related to Bröhm et al.: Methylation of recombinant mononucleosomes by DNMT3A demonstrates efficient linker DNA methylation and a role of H3K36me3

Nov 25, 2021 - Amplicon based bisulfite NGS data

Jeltsch, Albert; Bashtrykov, Pavel; Bröhm, Alexander; Dukatz, Michael; Adam, Sabrina, 2021, "NGS data related to Bröhm et al.: Methylation of recombinant mononucleosomes by DNMT3A demonstrates efficient linker DNA methylation and a role of H3K36me3", https://doi.org/10.18419/DARUS-1252, DaRUS, V2

Methylation experiments: For the competitive nucleosome methylation experiments, 0.6 pmol of each nucleosome variant were digested with MluI (NEB) for 60 min at 37°C in 10 µL NEB Cutsmart buffer (50 mM KOAc/20 mM Tris-acetate pH 7.9, 10 mM Magnesium Acetate, 100 µg/mL BSA) to remove residual unbound DNA. Afterwards, DNMT3A2 or DNMT3AC was added to...

DNMT3A2_Nuc_K4me3_R1x.fastqsanger

Nov 25, 2021 - NGS data related to Bröhm et al.: Methylation of recombinant mononucleosomes by DNMT3A demonstrates efficient linker DNA methylation and a role of H3K36me3

Unknown - 104.0 MB -

DNMT3A2_Nuc_K4me3_R2x.fastqsanger

Nov 25, 2021 - NGS data related to Bröhm et al.: Methylation of recombinant mononucleosomes by DNMT3A demonstrates efficient linker DNA methylation and a role of H3K36me3

Unknown - 28.3 MB -

DNMT3A2_Nuc_K4me3_R3x.fastqsanger

Nov 25, 2021 - NGS data related to Bröhm et al.: Methylation of recombinant mononucleosomes by DNMT3A demonstrates efficient linker DNA methylation and a role of H3K36me3

Unknown - 53.8 MB -

DNMT3A2_Nuc_unmod_R1x.fastqsanger

Nov 25, 2021 - NGS data related to Bröhm et al.: Methylation of recombinant mononucleosomes by DNMT3A demonstrates efficient linker DNA methylation and a role of H3K36me3

Unknown - 38.5 MB -