Institute of Biochemistry

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1,321 to 1,330 of 1,362 Results

Repeat 2, 3 min.txt Jan 30, 2020 - Deep enzymology data related to Adam et al.: DNA sequence-dependent activity and base flipping mechanisms of DNMT1 regulate genome-wide DNA methylation Plain Text - 19.1 MB - MD5: afe5285694973f2ba2aca8599ad7d514 Data
unmethylated upper strand.txt Jan 30, 2020 - Deep enzymology data related to Adam et al.: DNA sequence-dependent activity and base flipping mechanisms of DNMT1 regulate genome-wide DNA methylation Plain Text - 21.2 MB - MD5: aab989208e1cf78131f1a7b6c6e1c97b Data
Deep enzymology data related to Adam et al.: DNA sequence-dependent activity and base flipping mechanisms of DNMT1 regulate genome-wide DNA methylation Jan 30, 2020 - Amplicon based bisulfite NGS data Jeltsch, Albert; Bashtrykov, Pavel; Adam, Sabrina, 2020, "Deep enzymology data related to Adam et al.: DNA sequence-dependent activity and base flipping mechanisms of DNMT1 regulate genome-wide DNA methylation", https://doi.org/10.18419/DARUS-628, DaRUS, V1 Methylation of random flank substrates: Libraries of double stranded DNA substrates with unmethylated or hemimethylated CpG sites in randomized sequence context were methlyated by DNMT1. Reactions were stopped by shock freezing in liquid nitrogen, then treated with proteinase K for 2 hours. Afterwards, the DNA was digested with the BsaI-HFv2 enzyme...
hemimethylated Repeat 1.txt Jan 30, 2020 - Deep enzymology data related to Adam et al.: DNA sequence-dependent activity and base flipping mechanisms of DNMT1 regulate genome-wide DNA methylation Plain Text - 279.0 MB - MD5: cd22d0691983c4b6984a55bff3b4a543 Data
hemimethylated Repeat 2.txt Jan 30, 2020 - Deep enzymology data related to Adam et al.: DNA sequence-dependent activity and base flipping mechanisms of DNMT1 regulate genome-wide DNA methylation Plain Text - 17.6 MB - MD5: 5a481ba428f48c1afa51e5e707391a58 Data
hemimethylated without enzyme.txt Jan 30, 2020 - Deep enzymology data related to Adam et al.: DNA sequence-dependent activity and base flipping mechanisms of DNMT1 regulate genome-wide DNA methylation Plain Text - 18.5 MB - MD5: e0d3d3f8b1db1ec19e7e00bd55140672 Data
readme_random_flank_substrate.pdf Jan 30, 2020 - Deep enzymology data related to Adam et al.: DNA sequence-dependent activity and base flipping mechanisms of DNMT1 regulate genome-wide DNA methylation Adobe PDF - 436.1 KB - MD5: e854c1b0fe3d2d266f58f7538c6fb741 Documentation Additional information
unmethylated with enzyme.txt Jan 30, 2020 - Deep enzymology data related to Adam et al.: DNA sequence-dependent activity and base flipping mechanisms of DNMT1 regulate genome-wide DNA methylation Plain Text - 21.9 MB - MD5: 5f204000cefbfd71e59deef2357daba1 Data
unmethylated without enzyme.txt Jan 30, 2020 - Deep enzymology data related to Adam et al.: DNA sequence-dependent activity and base flipping mechanisms of DNMT1 regulate genome-wide DNA methylation Plain Text - 17.5 MB - MD5: 8bf6eb4956113e9ad601001f28052d65 Data
CBM63sequences.tab Jan 30, 2020 - Expansin domains in CBM63 sequences Tabular Data - 8.1 KB - 1 Variables, 510 Observations - UNF:6:9nlqS8HIWJ5ngSIjGG58OQ== Tabular file with headers: CBM63 with both domains (1st column), CBM63 with N-terminal domain (2nd column), CBM63 with C-terminal domain (3rd column). The expansin domains were annotated using a minimal domain-based score of 35.

Repeat 2, 3 min.txt

Jan 30, 2020 - Deep enzymology data related to Adam et al.: DNA sequence-dependent activity and base flipping mechanisms of DNMT1 regulate genome-wide DNA methylation

Plain Text - 19.1 MB -

Data

unmethylated upper strand.txt

Jan 30, 2020 - Deep enzymology data related to Adam et al.: DNA sequence-dependent activity and base flipping mechanisms of DNMT1 regulate genome-wide DNA methylation

Plain Text - 21.2 MB -

Data

Deep enzymology data related to Adam et al.: DNA sequence-dependent activity and base flipping mechanisms of DNMT1 regulate genome-wide DNA methylation

Jan 30, 2020 - Amplicon based bisulfite NGS data

Jeltsch, Albert; Bashtrykov, Pavel; Adam, Sabrina, 2020, "Deep enzymology data related to Adam et al.: DNA sequence-dependent activity and base flipping mechanisms of DNMT1 regulate genome-wide DNA methylation", https://doi.org/10.18419/DARUS-628, DaRUS, V1

Methylation of random flank substrates: Libraries of double stranded DNA substrates with unmethylated or hemimethylated CpG sites in randomized sequence context were methlyated by DNMT1. Reactions were stopped by shock freezing in liquid nitrogen, then treated with proteinase K for 2 hours. Afterwards, the DNA was digested with the BsaI-HFv2 enzyme...

hemimethylated Repeat 1.txt

Jan 30, 2020 - Deep enzymology data related to Adam et al.: DNA sequence-dependent activity and base flipping mechanisms of DNMT1 regulate genome-wide DNA methylation

Plain Text - 279.0 MB -

Data

hemimethylated Repeat 2.txt

Jan 30, 2020 - Deep enzymology data related to Adam et al.: DNA sequence-dependent activity and base flipping mechanisms of DNMT1 regulate genome-wide DNA methylation

Plain Text - 17.6 MB -

Data

hemimethylated without enzyme.txt

Jan 30, 2020 - Deep enzymology data related to Adam et al.: DNA sequence-dependent activity and base flipping mechanisms of DNMT1 regulate genome-wide DNA methylation

Plain Text - 18.5 MB -

Data

readme_random_flank_substrate.pdf

Jan 30, 2020 - Deep enzymology data related to Adam et al.: DNA sequence-dependent activity and base flipping mechanisms of DNMT1 regulate genome-wide DNA methylation

Adobe PDF - 436.1 KB -

Documentation

Additional information

unmethylated with enzyme.txt

Jan 30, 2020 - Deep enzymology data related to Adam et al.: DNA sequence-dependent activity and base flipping mechanisms of DNMT1 regulate genome-wide DNA methylation

Plain Text - 21.9 MB -

Data

unmethylated without enzyme.txt

Jan 30, 2020 - Deep enzymology data related to Adam et al.: DNA sequence-dependent activity and base flipping mechanisms of DNMT1 regulate genome-wide DNA methylation

Plain Text - 17.5 MB -

Data

CBM63sequences.tab

Jan 30, 2020 - Expansin domains in CBM63 sequences

Tabular Data - 8.1 KB - 1 Variables, 510 Observations -

Tabular file with headers: CBM63 with both domains (1st column), CBM63 with N-terminal domain (2nd column), CBM63 with C-terminal domain (3rd column). The expansin domains were annotated using a minimal domain-based score of 35.

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