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PNG Image - 684.6 KB - MD5: b5f1ea219de639537d315f158e16546c
Image with the observed crack pattern
PNG Image - 656.5 KB - MD5: 3907402df3401df71e249198a2caf1ff
Image with the observed crack pattern
PNG Image - 664.3 KB - MD5: 829c9d797dbf09162150a9c2db286c2f
Image with the observed crack pattern
Comma Separated Values - 7.8 KB - MD5: a4d1840cf63d938d4f2f8bd37f19a2dd
Values for the load-deformation curve of the numerical simulation used to perform the described mesh study
MS Excel Spreadsheet - 126.4 KB - MD5: cef56568e0a981040a4c60342431e5f4
Values for the load-deformation curve of the numerical simulation used to perform the described mesh study
Comma Separated Values - 25.5 KB - MD5: e6e05d4f209e4708e152ac63889170f4
Values for the load-deformation curve of the numerical simulation used to perform the described viscosity study
MS Excel Spreadsheet - 139.4 KB - MD5: 34737a48172d951a235490a8495f5050
Values for the load-deformation curve of the numerical simulation used to perform the described viscosity study
Jan 10, 2024 - Amplicon based bisulfite NGS data
Jeltsch, Albert; Bashtrykov, Pavel; Albrecht, Claudia, 2024, "NGS data related to Albrecht et al.: Locus specific and stable DNA demethylation at the H19/IGF2 ICR1 by epigenome editing using a dCas9-SunTag system and the catalytic domain of TET1", https://doi.org/10.18419/DARUS-3790, DaRUS, V1
Method overview For targeted DNA demethylation of the H19/IGF2 ICR1, HEK293 cells were transfected with two plasmids, one containing dCas9 fused to a SunTag with five repeats of the GCN4 peptide, separated by 22 aa long linkers, and scFv-fused TET1CD, as well as a GFP reporter protein. The second plasmid encodes five sgRNAs targeting the ICR1 and a...
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