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MS Excel Spreadsheet - 139.4 KB -
MD5: 34737a48172d951a235490a8495f5050
Values for the load-deformation curve of the numerical simulation used to perform the described viscosity study |
Jan 10, 2024 - Amplicon based bisulfite NGS data
Jeltsch, Albert; Bashtrykov, Pavel; Albrecht, Claudia, 2024, "NGS data related to Albrecht et al.: Locus specific and stable DNA demethylation at the H19/IGF2 ICR1 by epigenome editing using a dCas9-SunTag system and the catalytic domain of TET1", https://doi.org/10.18419/DARUS-3790, DaRUS, V1
Method overview For targeted DNA demethylation of the H19/IGF2 ICR1, HEK293 cells were transfected with two plasmids, one containing dCas9 fused to a SunTag with five repeats of the GCN4 peptide, separated by 22 aa long linkers, and scFv-fused TET1CD, as well as a GFP reporter protein. The second plasmid encodes five sgRNAs targeting the ICR1 and a... |
Gzip Archive - 57.5 MB -
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MD5: 9931bec183b91255eea8ab7c7c0965d9
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MD5: 7993fabe38431b573aa1ba315380437d
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MD5: 58141477909cac5dda5823cbe7ef00a1
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