HPLC raw data related to Koeppl et al.: Structure, function and substrate preferences of archaeal S-adenosyl-L-homocysteine hydrolases

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Koeppl, Lars-Hendrik, 2024, "HPLC raw data related to Koeppl et al.: Structure, function and substrate preferences of archaeal S-adenosyl-L-homocysteine hydrolases", https://doi.org/10.18419/darus-4052, DaRUS, V1, UNF:6:WOWb9VZ7dRJmQmW8TZ5lMg== [fileUNF]

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Description	In this study, synthesis and cleavage reactions catalysed by S-adenosyl-L-homocysteine hydrolases originating from different domains of life were investigated. The reactions were studied using S-adenosyl-L-homocysteine or S-inosyl-L-homocysteine as substrate for the cleavage reaction, and L-homocysteine together with inosine or adenosine as substrates for the synthesis reaction. The data contains the underlying HPLC chromatograms shown in Figures 3b and 3c as well as the Supplementary Figures S4-S24. All chromatograms are stored within the .xlsx file. The retention times (in min) are given in column A and the absorption values (in mAU) are given in column B. Additionally, the csv folder contains all data sheets of the excel file as individual .csv files. (2024-02-26)
Subject	Chemistry; Medicine, Health and Life Sciences
License/Data Use Agreement	CC BY 4.0

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	Supplementary Data 1.xlsx MS Excel Spreadsheet - 13.1 MB Published Mar 28, 2024 15 Downloads MD5: 00adf77587fdd5d0fc18b6165b53eae0 Source data underlying the HPLC chromatograms shown in the main Figs. 3b and 3c as well as the Supplementary Figs. S4-S24.	Access File File Access Public Download Options MS Excel Spreadsheet Download Metadata Data File Citation EndNote XML RIS BibTeX
	Fig3A_HSMT.tab csv/Tabular Data - 93.5 KB Published Mar 28, 2024 9 Downloads 2 Variables, 2999 Observations UNF:6:h6umKx9fUqErvatYVSFmWA==	Preview "csv/Fig3A_HSMT.tab" Access File File Access Public Download Options Comma Separated Values (Original File Format) Tab-Delimited RData Download Metadata Variable Metadata Data File Citation EndNote XML RIS BibTeX
	Fig3A_Negative.tab csv/Tabular Data - 93.6 KB Published Mar 28, 2024 14 Downloads 2 Variables, 3000 Observations UNF:6:yKMnCXc7PXbjXHoXw/wUug==	Preview "csv/Fig3A_Negative.tab" Access File File Access Public Download Options Comma Separated Values (Original File Format) Tab-Delimited RData Download Metadata Variable Metadata Data File Citation EndNote XML RIS BibTeX
	Fig3A_SIH.tab csv/Tabular Data - 93.6 KB Published Mar 28, 2024 9 Downloads 2 Variables, 3000 Observations UNF:6:NYXblwuBe3ZlJ3gok4NyYw==	Preview "csv/Fig3A_SIH.tab" Access File File Access Public Download Options Comma Separated Values (Original File Format) Tab-Delimited RData Download Metadata Variable Metadata Data File Citation EndNote XML RIS BibTeX
	Fig3B_HSMT.tab csv/Tabular Data - 94.1 KB Published Mar 28, 2024 16 Downloads 2 Variables, 3000 Observations UNF:6:jguO/IqBzqP1H7z8Ar26jg==	Preview "csv/Fig3B_HSMT.tab" Access File File Access Public Download Options Comma Separated Values (Original File Format) Tab-Delimited RData Download Metadata Variable Metadata Data File Citation EndNote XML RIS BibTeX
	Fig3B_Negative.tab csv/Tabular Data - 95.2 KB Published Mar 28, 2024 14 Downloads 2 Variables, 3000 Observations UNF:6:Cg1yh6OTRQRCTR+Lfq+x3g==	Preview "csv/Fig3B_Negative.tab" Access File File Access Public Download Options Comma Separated Values (Original File Format) Tab-Delimited RData Download Metadata Variable Metadata Data File Citation EndNote XML RIS BibTeX
	Fig3B_SAH.tab csv/Tabular Data - 95.3 KB Published Mar 28, 2024 19 Downloads 2 Variables, 3000 Observations UNF:6:jqihLLNs+6d+PoTjzZZ8iw==	Preview "csv/Fig3B_SAH.tab" Access File File Access Public Download Options Comma Separated Values (Original File Format) Tab-Delimited RData Download Metadata Variable Metadata Data File Citation EndNote XML RIS BibTeX
	S10AA_Ado.tab csv/Tabular Data - 93.7 KB Published Mar 28, 2024 20 Downloads 2 Variables, 3000 Observations UNF:6:rl5YZghU33/pW0xt56XoSQ==	Preview "csv/S10AA_Ado.tab" Access File File Access Public Download Options Comma Separated Values (Original File Format) Tab-Delimited RData Download Metadata Variable Metadata Data File Citation EndNote XML RIS BibTeX
	S10AA_Negative.tab csv/Tabular Data - 93.7 KB Published Mar 28, 2024 4 Downloads 2 Variables, 3000 Observations UNF:6:c0aQqKP66/MsLWcMOEbfGQ==	Preview "csv/S10AA_Negative.tab" Access File File Access Public Download Options Comma Separated Values (Original File Format) Tab-Delimited RData Download Metadata Variable Metadata Data File Citation EndNote XML RIS BibTeX
	S10AB_HSMT.tab csv/Tabular Data - 94.4 KB Published Mar 28, 2024 18 Downloads 2 Variables, 3000 Observations UNF:6:bFveGJkuPM0UNyrUn5opfg==	Preview "csv/S10AB_HSMT.tab" Access File File Access Public Download Options Comma Separated Values (Original File Format) Tab-Delimited RData Download Metadata Variable Metadata Data File Citation EndNote XML RIS BibTeX

Citation Metadata

Persistent Identifier	doi:10.18419/darus-4052
Publication Date	2024-03-28
Title	HPLC raw data related to Koeppl et al.: Structure, function and substrate preferences of archaeal S-adenosyl-L-homocysteine hydrolases
Author	Koeppl, Lars-Hendrik (Albert-Ludwigs-Universität Freiburg) - ORCID: 0000-0001-6222-2125
Point of Contact	Use email button above to contact. Koeppl, Lars-Hendrik (Albert-Ludwigs-Universität Freiburg) Andexer, Jennifer N. (Albert-Ludwigs-Universität Freiburg)
Description	In this study, synthesis and cleavage reactions catalysed by S-adenosyl-L-homocysteine hydrolases originating from different domains of life were investigated. The reactions were studied using S-adenosyl-L-homocysteine or S-inosyl-L-homocysteine as substrate for the cleavage reaction, and L-homocysteine together with inosine or adenosine as substrates for the synthesis reaction. The data contains the underlying HPLC chromatograms shown in Figures 3b and 3c as well as the Supplementary Figures S4-S24. All chromatograms are stored within the .xlsx file. The retention times (in min) are given in column A and the absorption values (in mAU) are given in column B. Additionally, the csv folder contains all data sheets of the excel file as individual .csv files. (2024-02-26)
Subject	Chemistry; Medicine, Health and Life Sciences
Funding Information	DFG: 235777276 - RTG1976 DFG: 527572100 European Commission: info:eu-repo/grantAgreement/EC/H2020/ERC/716966
Depositor	Koeppl, Lars-Hendrik
Deposit Date	2024-02-26

EnzymeML

Vessels	reaction tube : 1.5 [ml] Constant
Reactants	s0 S-adenosyl-L-homocysteine : 200 [uM] Not constant InChI: InChI=1S/C14H20N6O5S/c15-6(14(23)24)1-2-26-3-7-9(21)10(22)13(25-7)20-5-19-8-11(16)17-4-18-12(8)20/h4-7,9-10,13,21-22H,1-3,15H2,(H,23,24)(H2,16,17,18)/t6-,7+,9+,10+,13+/m0/s1 s1 S-inosyl-L-homocysteine : 200 [uM] Not constant InChI: InChI=1S/C14H19N5O6S/c15-6(14(23)24)1-2-26-3-7-9(20)10(21)13(25-7)19-5-18-8-11(19)16-4-17-12(8)22/h4-7,9-10,13,20-21H,1-3,15H2,(H,23,24)(H,16,17,22)/t6-,7+,9+,10+,13+/m0/s1 s2 adenosine : 500 [uM] Not constant InChI: InChI=1S/C10H13N5O4/c11-8-5-9(13-2-12-8)15(3-14-5)10-7(18)6(17)4(1-16)19-10/h2-4,6-7,10,16-18H,1H2,(H2,11,12,13)/t4-,6-,7-,10-/m1/s1 s3 inosine : 500 [uM] Not constant InChI: InChI=1S/C10H12N4O5/c15-1-4-6(16)7(17)10(19-4)14-3-13-5-8(14)11-2-12-9(5)18/h2-4,6-7,10,15-17H,1H2,(H,11,12,18)/t4-,6-,7-,10-/m1/s1 s4 L-homocysteine : 500 [uM] Not constant InChI: InChI=1S/C4H9NO2S/c5-3(1-2-8)4(6)7/h3,8H,1-2,5H2,(H,6,7)/t3-/m0/s1
Proteins	p0 S-adenosyl-l-homocysteine hydrolase : 5 [uM] : Constant 3.13.2.1
Reactions	: SAH cleavage 37 [Celsius] 7.5 ; educts: s0 ; products: s2, s4 ; modifier: p0 : SIH cleavage 37 [Celsius] 7.5 ; educts: s1 ; products: s3, s4 ; modifier: p0 : SAH synthesis 37 [Celsius] 7.5 ; educts: s2, s4 ; products: s0 ; modifier: p0 : SIH synthesis 37 [Celsius] 7.5 ; educts: s3, s4 ; products: s1 ; modifier: p0

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