Data for: Bacterial R-bodies with common morphologies and unrolling dynamics are phylogenetically scattered indicating extensive lateral gene transfer and wide application potential

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Schweikert, Michael; Dörr, Lennart; Ghosh, Robin, 2026, "Data for: Bacterial R-bodies with common morphologies and unrolling dynamics are phylogenetically scattered indicating extensive lateral gene transfer and wide application potential", https://doi.org/10.18419/DARUS-6419, DaRUS, V1
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Data repository of raw data used in: "Bacterial R-bodies with common morphologies and unrolling dynamics are phylogenetically scattered indicating extensive lateral gene transfer and wide application potential."

FIGURE S1 Rectangular phylogenetic maximum likelihood tree based on sequences of 62 different bacterial strains with homology to RebB from Cb. taeniospiralis. Reb proteins of the species studied here are highlighted. Numbers on branches show ultra-fast bootstrap values. Scale bar: the estimated proportional sequence divergence. Abbreviations: ct, Cb. taeniospiralis; cv, Cm. varicaedens; ra, Ro. alexandrii; pc, Ps. chlororaphis; aa, Ac. avenae; ht, Hy. taeniospiralis; mm, Ma. mediterranea.

FIGURE S2 Transmission electron microscopy images. (A) Tubular phage-like structures in the Ps. chlororaphis isolate. (B) Ps. chlororaphis R-body with extreme height. (C) Transition between two distinct layers (arrow) seen on an R-body isolated from Ps. chlororaphis. (D) to (F) Ultra-thin sections of high-pressure frozen Cm. varicaedens cells with R-bodies in the macronucleus of P. caudatum. The white arrows mark the sheath. The orange arrow marks where either the sheath or the outer R-body lamella appears connected to the cell membrane.

TABLE S1 Complete list of protein sequences used for the phylogenetic analysis. Sequences of the seven species used in this paper are highlighted in bold. Abbr. = abbreviation, PB = proteobacteria, CB = cyanobacteria, FB = flavobacteria.

VIDEO S1A Light microscopic video recording of acid-induced unrolling of an R-body isolated from Cm. varicaedens. The video was recorded in Full HD with 120 frames per second, slowed down 4x and scaled to 1,080 px.

VIDEO S1B Light microscopic video recording of alkali-induced unrolling of an R-body isolated from Cm. varicaedens. The video was recorded in Full HD with 120 frames per second, slowed down 4x and scaled to 1,080 px.

VIDEO S1C Light microscopic video recording of alkali-induced unrolling of an R-body isolated from Cm. varicaedens. The video was recorded in 4K Crop with 60 frames per second and scaled to 1,080 px.

VIDEO S1D Light microscopic video recording of acid-induced unrolling of an R-body isolated from Ro. alexandrii. The video was recorded in 4K Crop with 60 frames per second and scaled to 1,080 px.

VIDEO S1E Light microscopic video recording of acid-induced unrolling of an R-body isolated from Ps. chlororaphis. The video was recorded in Full HD with 120 frames per second, slowed down 4x and scaled to 1,080 px.

VIDEO S1F Light microscopic video recording of acid-induced unrolling of an R-body isolated from Ac. avenae. The video was recorded in Full HD with 120 frames per second, slowed down 4x and scaled to 1,080 px.

Supplementary Materials & Methods
Detailed procedures for the isolation of R-bodies and preparation for electron microscopy.

Caedibacter_var_Contig105_rc-RebA_combined_EMBL Complementary sequence rebABCDEF operon of Caedimonas varicaedens on contig_Cva_contig000105. Combines contig 105 data with PCR-derived data (2026-08-18)
Subject Earth and Environmental Sciences
Keyword R-Bodies, Inclusion Bodies, Gram-Negative Bacteria, Electron Microscopy, Phylogenetics
Related Publication Is Supplement To: Applied and Environmental Microbiology (2026) in press
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Caedibacter_var_Contig105_rc-RebA_combined_EMBL.txt
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Curation Status History
StatusDateAssigner
2026-08-25 06:34:14Michael Rolf Schweikert
Waiting for Feedback from Author2026-08-24 14:30:08Dataverse Admin
In Review by FoKUS2026-08-21 16:07:53Michael Rolf Schweikert
Waiting for Feedback from Author2026-08-20 14:57:13Dataverse Admin
In Review by FoKUS2026-08-20 05:56:33Dataverse Admin
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