NGS data related to Dukatz et al.: DNA methyltransferase DNMT3A forms interaction networks with the CpG site and flanking sequence elements for efficient methylation (doi:10.18419/darus-2993)

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NGS data related to Dukatz et al.: DNA methyltransferase DNMT3A forms interaction networks with the CpG site and flanking sequence elements for efficient methylation

doi:10.18419/darus-2993

DaRUS

2022-09-27

1

Jeltsch, Albert; Bashtrykov, Pavel; Dukatz, Michael; Adam, Sabrina, 2022, "NGS data related to Dukatz et al.: DNA methyltransferase DNMT3A forms interaction networks with the CpG site and flanking sequence elements for efficient methylation", https://doi.org/10.18419/DARUS-2993, DaRUS, V1

NGS data related to Dukatz et al.: DNA methyltransferase DNMT3A forms interaction networks with the CpG site and flanking sequence elements for efficient methylation

doi:10.18419/darus-2993

Jeltsch, Albert (Universität Stuttgart)

Bashtrykov, Pavel (Universität Stuttgart)

Dukatz, Michael (Universität Stuttgart)

Adam, Sabrina (Universität Stuttgart)

JE 252/10 - 194537093

JE 252/36 - 403074082

DaRUS

Jeltsch, Albert

Jeltsch, Albert

2022-06-15

https://doi.org/10.18419/DARUS-2993

Chemistry, Medicine, Health and Life Sciences, DNA Methylation, DNA Methyltransferase, NGS Bisulfite Sequencing, Bisulfite Sequencing, Enzyme Assay, DNMT3A

Biochemistry

The naming of the files is described in the Supplemental Tables 1 of the accompanying manuscript.

Raw DNA sequences in Fastqsanger format

Bisulfite-seq DNA methylation analysis

Dukatz et al.: "DNA methyltransferase DNMT3A forms interaction networks with the CpG site and flanking sequence elements for efficient methylation", Journal of Biological Chemistry, 2022

10.1016/j.jbc.2022.102462

Dukatz et al.: "DNA methyltransferase DNMT3A forms interaction networks with the CpG site and flanking sequence elements for efficient methylation", Journal of Biological Chemistry, 2022

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readme.pdf

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MBP-DNMT3A WT CG 2 µM_R1.txt

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MBP-DNMT3A WT CG 4 µM_R2.txt

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MBP-L883A CG 0.5 µM_R1.txt

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MBP-L883A CG 1 µM_R2.txt

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MBP-N838A CG 2 µM_R1.txt

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MBP-N838A CG 2µM_R2.txt

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MBP-R836A CG 3 µM_R1.txt

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MBP-R836A CG 6 µM_R2.txt

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MBP-R882A CG 10 µM_R1.txt

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MBP-R887A CG 2 µM_R1.txt

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MBP-R887A CG 4 µM_R2.txt

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MBP-S837A CG 2 µM_R1.txt

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MBP-S837A CG 4 µM_R2.txt

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MBP-S881A CG 3 µM_R1.txt

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MBP-S881A CG 6 µM_R2.txt

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MBP_cleaved_R882A 4 µM CG_R2.txt

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no enzyme control CG substrate.txt

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0.5 µM DNMT3A L883A 120 min CN sub.txt

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0.5 µM DNMT3A L883A 30 min CN sub.txt

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2 µM DNMT3A N838A CD on CN 30 min.txt

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2 µM DNMT3A N838A CD on CN 60 min.txt

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2 µM DNMT3A R887A 120 min CN sub.txt

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2 µM DNMT3A R887A 30 min CN sub.txt

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2 µM DNMT3A WT 120 min CN sub.txt

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2 µM DNMT3A WT 30 min CN sub.txt

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4 µM DNMT3A R836A 120 min CN sub.txt

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4 µM DNMT3A R836A 30 min CN sub.txt

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4 µM DNMT3A R882A 120 min CN sub.txt

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4 µM DNMT3A R882A 30 min CN sub.txt

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4 µM DNMT3A R882H 120 min CN sub.txt

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4 µM DNMT3A R882H 30 min CN sub.txt

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4 µM DNMT3A S837A 120 min CN sub.txt

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4 µM DNMT3A S837A 30 min CN sub.txt

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4 µM DNMT3A S881A 120 min CN sub.txt

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4 µM DNMT3A S881A 30 min CN sub.txt

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8 µM DNMT3A S881D 120 min CN sub.txt

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8 µM DNMT3A S881D 30 min CN sub.txt

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no enzyme control CN substrate.txt

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0.5 µM DNMT3A L883A CD on MD_221 120 min.tabular

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0.5 µM DNMT3A L883A CD on MD_221 30 min.tabular

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0.5 µM DNMT3A L883A CD on MD_222 120 min.tabular

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0.5 µM DNMT3A L883A CD on MD_222 30 min.tabular

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0.5 µM DNMT3A L883A CD on PB_954 120 min.tabular

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0.5 µM DNMT3A L883A CD on PB_954 30 min.tabular

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0.5 µM DNMT3A L883A CD on PB_955 120 min.tabular

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0.5 µM DNMT3A L883A CD on PB_955 30 min.tabular

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0.5 µM DNMT3A L883A CD on PB_956 120 min.tabular

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0.5 µM DNMT3A L883A CD on PB_956 30 min.tabular

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0.5 µM DNMT3A WT CD 60 min on MD_221.txt

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0.5 µM DNMT3A WT CD 60 min on MD_222.txt

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0.5 µM DNMT3A WT CD 60 min on PB_954.txt

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0.5 µM DNMT3A WT CD 60 min on PB_955.txt

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0.5 µM DNMT3A WT CD 60 min on PB_956.txt

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1 µM DNMT3A WT CD 60 min on MD_221.txt

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1 µM DNMT3A WT CD 60 min on MD_222.txt

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1 µM DNMT3A WT CD 60 min on PB_954.txt

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1 µM DNMT3A WT CD 60 min on PB_955.txt

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1 µM DNMT3A WT CD 60 min on PB_956.txt

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2 µM DNMT3A S837A CD on MD_221 120 min.tabular

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2 µM DNMT3A S837A CD on MD_221 30 min.tabular

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2 µM DNMT3A S837A CD on MD_222 120 min.tabular

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2 µM DNMT3A S837A CD on MD_222 30 min.tabular

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2 µM DNMT3A S837A CD on PB_954 120 min.tabular

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2 µM DNMT3A S837A CD on PB_954 30 min.tabular

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2 µM DNMT3A S837A CD on PB_955 120 min.tabular

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2 µM DNMT3A S837A CD on PB_955 30 min.tabular

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2 µM DNMT3A S837A CD on PB_956 120 min.tabular

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2 µM DNMT3A S837A CD on PB_956 30 min.tabular

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2 µM DNMT3A S881A CD on MD_221 120 min.tabular

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2 µM DNMT3A S881A CD on MD_221 30 min.tabular

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2 µM DNMT3A S881A CD on MD_222 120 min.tabular

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2 µM DNMT3A S881A CD on MD_222 30 min.tabular

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2 µM DNMT3A S881A CD on PB_954 120 min.tabular

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2 µM DNMT3A S881A CD on PB_954 30 min.tabular

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2 µM DNMT3A S881A CD on PB_955 120 min.tabular

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2 µM DNMT3A S881A CD on PB_955 30 min.tabular

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2 µM DNMT3A S881A CD on PB_956 120 min.tabular

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2 µM DNMT3A S881A CD on PB_956 30 min.tabular

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3 µM DNMT3A R836A CD on MD_221 120 min.tabular

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3 µM DNMT3A R836A CD on MD_221 30 min.tabular

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3 µM DNMT3A R836A CD on MD_222 120 min.tabular

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3 µM DNMT3A R836A CD on MD_222 30 min.tabular

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3 µM DNMT3A R836A CD on PB_954 120 min.tabular

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3 µM DNMT3A R836A CD on PB_954 30 min.tabular

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3 µM DNMT3A R836A CD on PB_955 120 min.tabular

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3 µM DNMT3A R836A CD on PB_955 30 min.tabular

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3 µM DNMT3A R836A CD on PB_956 120 min.tabular

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Label:

3 µM DNMT3A R836A CD on PB_956 30 min.tabular

application/octet-stream

Label:

3 µM DNMT3A R887A CD on MD_221 120 min.tabular

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3 µM DNMT3A R887A CD on MD_221 30 min.tabular

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3 µM DNMT3A R887A CD on MD_222 120 min.tabular

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3 µM DNMT3A R887A CD on MD_222 30 min.tabular

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3 µM DNMT3A R887A CD on PB_954 120 min.tabular

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3 µM DNMT3A R887A CD on PB_954 30 min.tabular

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3 µM DNMT3A R887A CD on PB_955 120 min.tabular

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3 µM DNMT3A R887A CD on PB_955 30 min.tabular

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3 µM DNMT3A R887A CD on PB_956 120 min.tabular

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3 µM DNMT3A R887A CD on PB_956 30 min.tabular

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4 µM DNMT3A R882A CD on MD_221 120 min.tabular

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4 µM DNMT3A R882A CD on MD_221 30 min.tabular

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4 µM DNMT3A R882A CD on MD_222 120 min.tabular

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4 µM DNMT3A R882A CD on MD_222 30 min.tabular

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4 µM DNMT3A R882A CD on PB_954 120 min.tabular

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4 µM DNMT3A R882A CD on PB_954 30 min.tabular

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Label:

4 µM DNMT3A R882A CD on PB_955 120 min.tabular

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4 µM DNMT3A R882A CD on PB_955 30 min.tabular

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4 µM DNMT3A R882A CD on PB_956 120 min.tabular

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4 µM DNMT3A R882A CD on PB_956 30 min.tabular

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4 µM DNMT3A R882H CD on MD_221 120 min.tabular

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4 µM DNMT3A R882H CD on MD_221 30 min.tabular

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4 µM DNMT3A R882H CD on MD_222 120 min.tabular

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4 µM DNMT3A R882H CD on MD_222 30 min.tabular

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4 µM DNMT3A R882H CD on PB_954 120 min.tabular

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4 µM DNMT3A R882H CD on PB_954 30 min.tabular

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4 µM DNMT3A R882H CD on PB_955 120 min.tabular

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4 µM DNMT3A R882H CD on PB_955 30 min.tabular

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4 µM DNMT3A R882H CD on PB_956 120 min.tabular

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4 µM DNMT3A R882H CD on PB_956 30 min.tabular

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no enzyme control MD_221.txt

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no enzyme control MD_222.txt

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no enzyme control PB_954.txt

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no enzyme control PB_955.txt

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no enzyme control PB_956.txt

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