NGS data related to Dukatz et al.: DNA methyltransferase DNMT3A forms interaction networks with the CpG site and flanking sequence elements for efficient methylation (doi:10.18419/darus-2993)

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NGS data related to Dukatz et al.: DNA methyltransferase DNMT3A forms interaction networks with the CpG site and flanking sequence elements for efficient methylation

doi:10.18419/darus-2993

DaRUS

2022-09-27

1

Jeltsch, Albert; Bashtrykov, Pavel; Dukatz, Michael; Adam, Sabrina, 2022, "NGS data related to Dukatz et al.: DNA methyltransferase DNMT3A forms interaction networks with the CpG site and flanking sequence elements for efficient methylation", https://doi.org/10.18419/darus-2993, DaRUS, V1

NGS data related to Dukatz et al.: DNA methyltransferase DNMT3A forms interaction networks with the CpG site and flanking sequence elements for efficient methylation

doi:10.18419/darus-2993

Jeltsch, Albert (Universität Stuttgart)

Bashtrykov, Pavel (Universität Stuttgart)

Dukatz, Michael (Universität Stuttgart)

Adam, Sabrina (Universität Stuttgart)

JE 252/10 - 194537093

JE 252/36 - 403074082

DaRUS

Jeltsch, Albert

Jeltsch, Albert

2022-06-15

https://doi.org/10.18419/darus-2993

Chemistry, Medicine, Health and Life Sciences, DNA Methylation, DNA Methyltransferase, NGS Bisulfite Sequencing, Bisulfite Sequencing, Enzyme Assay, DNMT3A

The naming of the files is described in the Supplemental Tables 1 of the accompanying manuscript.

Raw DNA sequences in Fastqsanger format

Bisulfite-seq DNA methylation analysis

Dukatz et al.: "DNA methyltransferase DNMT3A forms interaction networks with the CpG site and flanking sequence elements for efficient methylation", Journal of Biological Chemistry, 2022

10.1016/j.jbc.2022.102462

Dukatz et al.: "DNA methyltransferase DNMT3A forms interaction networks with the CpG site and flanking sequence elements for efficient methylation", Journal of Biological Chemistry, 2022

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readme.pdf

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MBP-DNMT3A WT CG 2 µM_R1.txt

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MBP-DNMT3A WT CG 4 µM_R2.txt

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MBP-L883A CG 0.5 µM_R1.txt

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MBP-L883A CG 1 µM_R2.txt

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MBP-N838A CG 2 µM_R1.txt

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MBP-N838A CG 2µM_R2.txt

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MBP-R836A CG 3 µM_R1.txt

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MBP-R836A CG 6 µM_R2.txt

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MBP-R882A CG 10 µM_R1.txt

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MBP-R887A CG 2 µM_R1.txt

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MBP-R887A CG 4 µM_R2.txt

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MBP-S837A CG 2 µM_R1.txt

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MBP-S837A CG 4 µM_R2.txt

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MBP-S881A CG 3 µM_R1.txt

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MBP-S881A CG 6 µM_R2.txt

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MBP_cleaved_R882A 4 µM CG_R2.txt

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no enzyme control CG substrate.txt

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0.5 µM DNMT3A L883A 120 min CN sub.txt

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0.5 µM DNMT3A L883A 30 min CN sub.txt

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2 µM DNMT3A N838A CD on CN 30 min.txt

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2 µM DNMT3A N838A CD on CN 60 min.txt

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2 µM DNMT3A R887A 120 min CN sub.txt

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2 µM DNMT3A R887A 30 min CN sub.txt

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2 µM DNMT3A WT 120 min CN sub.txt

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2 µM DNMT3A WT 30 min CN sub.txt

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4 µM DNMT3A R836A 120 min CN sub.txt

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4 µM DNMT3A R836A 30 min CN sub.txt

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4 µM DNMT3A R882A 120 min CN sub.txt

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4 µM DNMT3A R882A 30 min CN sub.txt

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4 µM DNMT3A R882H 120 min CN sub.txt

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4 µM DNMT3A R882H 30 min CN sub.txt

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4 µM DNMT3A S837A 120 min CN sub.txt

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4 µM DNMT3A S837A 30 min CN sub.txt

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4 µM DNMT3A S881A 120 min CN sub.txt

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4 µM DNMT3A S881A 30 min CN sub.txt

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8 µM DNMT3A S881D 120 min CN sub.txt

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8 µM DNMT3A S881D 30 min CN sub.txt

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no enzyme control CN substrate.txt

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0.5 µM DNMT3A L883A CD on MD_221 120 min.tabular

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0.5 µM DNMT3A L883A CD on MD_221 30 min.tabular

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0.5 µM DNMT3A L883A CD on MD_222 120 min.tabular

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0.5 µM DNMT3A L883A CD on MD_222 30 min.tabular

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0.5 µM DNMT3A L883A CD on PB_954 120 min.tabular

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0.5 µM DNMT3A L883A CD on PB_954 30 min.tabular

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0.5 µM DNMT3A L883A CD on PB_955 120 min.tabular

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0.5 µM DNMT3A L883A CD on PB_955 30 min.tabular

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0.5 µM DNMT3A L883A CD on PB_956 120 min.tabular

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0.5 µM DNMT3A L883A CD on PB_956 30 min.tabular

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0.5 µM DNMT3A WT CD 60 min on MD_221.txt

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0.5 µM DNMT3A WT CD 60 min on MD_222.txt

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0.5 µM DNMT3A WT CD 60 min on PB_954.txt

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0.5 µM DNMT3A WT CD 60 min on PB_955.txt

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0.5 µM DNMT3A WT CD 60 min on PB_956.txt

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1 µM DNMT3A WT CD 60 min on MD_221.txt

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1 µM DNMT3A WT CD 60 min on MD_222.txt

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1 µM DNMT3A WT CD 60 min on PB_954.txt

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1 µM DNMT3A WT CD 60 min on PB_955.txt

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1 µM DNMT3A WT CD 60 min on PB_956.txt

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2 µM DNMT3A S837A CD on MD_221 120 min.tabular

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2 µM DNMT3A S837A CD on MD_221 30 min.tabular

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2 µM DNMT3A S837A CD on MD_222 120 min.tabular

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2 µM DNMT3A S837A CD on MD_222 30 min.tabular

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2 µM DNMT3A S837A CD on PB_954 120 min.tabular

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2 µM DNMT3A S837A CD on PB_954 30 min.tabular

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2 µM DNMT3A S837A CD on PB_955 120 min.tabular

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2 µM DNMT3A S837A CD on PB_955 30 min.tabular

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2 µM DNMT3A S837A CD on PB_956 120 min.tabular

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2 µM DNMT3A S837A CD on PB_956 30 min.tabular

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2 µM DNMT3A S881A CD on MD_221 120 min.tabular

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2 µM DNMT3A S881A CD on MD_221 30 min.tabular

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2 µM DNMT3A S881A CD on MD_222 120 min.tabular

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2 µM DNMT3A S881A CD on MD_222 30 min.tabular

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2 µM DNMT3A S881A CD on PB_954 120 min.tabular

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2 µM DNMT3A S881A CD on PB_954 30 min.tabular

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2 µM DNMT3A S881A CD on PB_955 120 min.tabular

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2 µM DNMT3A S881A CD on PB_955 30 min.tabular

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2 µM DNMT3A S881A CD on PB_956 120 min.tabular

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2 µM DNMT3A S881A CD on PB_956 30 min.tabular

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3 µM DNMT3A R836A CD on MD_221 120 min.tabular

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3 µM DNMT3A R836A CD on MD_221 30 min.tabular

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3 µM DNMT3A R836A CD on MD_222 120 min.tabular

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3 µM DNMT3A R836A CD on MD_222 30 min.tabular

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3 µM DNMT3A R836A CD on PB_954 120 min.tabular

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3 µM DNMT3A R836A CD on PB_954 30 min.tabular

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3 µM DNMT3A R836A CD on PB_955 120 min.tabular

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3 µM DNMT3A R836A CD on PB_955 30 min.tabular

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3 µM DNMT3A R836A CD on PB_956 120 min.tabular

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3 µM DNMT3A R836A CD on PB_956 30 min.tabular

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Label:

3 µM DNMT3A R887A CD on MD_221 120 min.tabular

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3 µM DNMT3A R887A CD on MD_221 30 min.tabular

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3 µM DNMT3A R887A CD on MD_222 120 min.tabular

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3 µM DNMT3A R887A CD on MD_222 30 min.tabular

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3 µM DNMT3A R887A CD on PB_954 120 min.tabular

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3 µM DNMT3A R887A CD on PB_954 30 min.tabular

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3 µM DNMT3A R887A CD on PB_955 120 min.tabular

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3 µM DNMT3A R887A CD on PB_955 30 min.tabular

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3 µM DNMT3A R887A CD on PB_956 120 min.tabular

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3 µM DNMT3A R887A CD on PB_956 30 min.tabular

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4 µM DNMT3A R882A CD on MD_221 120 min.tabular

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4 µM DNMT3A R882A CD on MD_221 30 min.tabular

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4 µM DNMT3A R882A CD on MD_222 120 min.tabular

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4 µM DNMT3A R882A CD on MD_222 30 min.tabular

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4 µM DNMT3A R882A CD on PB_954 120 min.tabular

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4 µM DNMT3A R882A CD on PB_954 30 min.tabular

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Label:

4 µM DNMT3A R882A CD on PB_955 120 min.tabular

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4 µM DNMT3A R882A CD on PB_955 30 min.tabular

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Label:

4 µM DNMT3A R882A CD on PB_956 120 min.tabular

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4 µM DNMT3A R882A CD on PB_956 30 min.tabular

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4 µM DNMT3A R882H CD on MD_221 120 min.tabular

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4 µM DNMT3A R882H CD on MD_221 30 min.tabular

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4 µM DNMT3A R882H CD on MD_222 120 min.tabular

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4 µM DNMT3A R882H CD on MD_222 30 min.tabular

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4 µM DNMT3A R882H CD on PB_954 120 min.tabular

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4 µM DNMT3A R882H CD on PB_954 30 min.tabular

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4 µM DNMT3A R882H CD on PB_955 120 min.tabular

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4 µM DNMT3A R882H CD on PB_955 30 min.tabular

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4 µM DNMT3A R882H CD on PB_956 120 min.tabular

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4 µM DNMT3A R882H CD on PB_956 30 min.tabular

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no enzyme control MD_221.txt

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no enzyme control MD_222.txt

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no enzyme control PB_954.txt

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no enzyme control PB_955.txt

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no enzyme control PB_956.txt

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