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Part 1: Document Description
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Citation |
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Title: |
Propioin Synthesis using Benzoin Aldolase |
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Identification Number: |
doi:10.18419/darus-2466 |
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Distributor: |
DaRUS |
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Date of Distribution: |
2022-02-15 |
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Version: |
1 |
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Bibliographic Citation: |
Spöring, Jan-Dirk, 2022, "Propioin Synthesis using Benzoin Aldolase", https://doi.org/10.18419/darus-2466, DaRUS, V1 |
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Citation |
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Title: |
Propioin Synthesis using Benzoin Aldolase |
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Identification Number: |
doi:10.18419/darus-2466 |
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Authoring Entity: |
Spöring, Jan-Dirk (Forschungszentrum Jülich) |
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Software used in Production: |
PyEnzyme |
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Software used in Production: |
BioCatHub |
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Grant Number: |
EXC 2075 - 390740016 |
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Distributor: |
DaRUS |
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Access Authority: |
Spöring, Jan-Dirk |
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Access Authority: |
Prof. Dr. Dörte Rother |
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Holdings Information: |
https://doi.org/10.18419/darus-2466 |
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Study Scope |
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Keywords: |
Chemistry, Medicine, Health and Life Sciences |
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Topic Classification: |
Biocatalysis, Enzymology, EnzymeML, Biotechnology |
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Abstract: |
Investigated was the ligation of two molecules propanal in organic conditions, catalysed by the benzoin aldolase, which was added in the form of lyophillised whole cells. The reaction was performed in triplicate, with 200 mM as the starting concentration of the propanal in all cases. Triethanolamine was chosen as buffer, together with Cyclopentyl methyl ether as the organic solvent, the experiment was performed at 30°C and at a pH of 9. The concentration of propioin was followed over the course of 2 hours. |
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Notes: |
The OMEX EnzymeML archive was created using the web-based electronic lab notebook BioCatHub (https://biocathub.net) and its EnzymeML export implementation. In addition, the appended notebook was used to model the data by utilizing the Python library <b>lmfit</b> in conjunction with <b>PyEnzyme</b>. For an inspection of the data it is advised to use the <a href="https://github.com/EnzymeML/PyEnzyme">PyEnzyme</a> library. |
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Methodology and Processing |
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Sources Statement |
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Data Access |
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Other Study Description Materials |
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Label: |
CollectingDataByBioCatHubAndVisualisation.ipynb |
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Text: |
Jupyter Notebook that was used to model the given OMEX EnzymeML archive |
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Notes: |
application/x-ipynb+json |
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Label: |
PropioinSynthesis_Spoering.omex |
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Text: | |
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Notes: |
text/plain |