Carboligation of Pyruvate catalysed by HcAHAS C Unit (doi:10.18419/darus-2470)

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Part 1: Document Description
Part 2: Study Description
Part 5: Other Study-Related Materials
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Document Description

Citation

Title:

Carboligation of Pyruvate catalysed by HcAHAS C Unit

Identification Number:

doi:10.18419/darus-2470

Distributor:

DaRUS

Date of Distribution:

2022-02-15

Version:

1

Bibliographic Citation:

Malzacher, Stephan, 2022, "Carboligation of Pyruvate catalysed by HcAHAS C Unit", https://doi.org/10.18419/darus-2470, DaRUS, V1

Study Description

Citation

Title:

Carboligation of Pyruvate catalysed by HcAHAS C Unit

Identification Number:

doi:10.18419/darus-2470

Authoring Entity:

Malzacher, Stephan (Forschungszentrum Jülich)

Software used in Production:

PyEnzyme

Grant Number:

EXC 2075 - 390740016

Distributor:

DaRUS

Access Authority:

Malzacher, Stephan

Holdings Information:

https://doi.org/10.18419/darus-2470

Study Scope

Keywords:

Chemistry, Medicine, Health and Life Sciences, Enzymology, Biocatalysis, Biotechnology, EnzymeML

Abstract:

<p>The data was measured in the course of a Bachelor thesis and filled into the EnzymeML Spreadsheet. </p> <p>Measured was the absorption for the reaction from pyruvate to acetolactate. The measurements were conducted for six different pH values (in the range from pH 5 to pH8). For each of these measurements, a different Spreadsheet was created.</p>

Notes:

This dataset includes the analysis of the enzyme kinetics at pH 7 for a demonstration of the workflow. The analysis can be reproduced by using the notebook attached to this dataset. In addition, the remaining pH studies were attached as spreadsheets, which can be converted using the <a href="https://enzymeml.sloppy.zone/#/EnzymeML%20spreadsheet/convert_template_template_convert_post">PyEnzyme REST-API</a>. In order to investigate the dataset, it is advised to use the <a href="https://github.com/EnzymeML/PyEnzyme">PyEnzyme</a> library.

Methodology and Processing

Sources Statement

Data Access

Other Study Description Materials

Other Study-Related Materials

Label:

AnalysingTimeCourseDataUsingMichaelisMenten.ipynb

Text:

Jupyter notebook that was used to model the OMEX archive found in this dataset.

Notes:

application/x-ipynb+json

Other Study-Related Materials

Label:

CarboligationOfPyruvateCatalysedByHcAHAS_C_unit_Malzacher.omex

Text:

Resulting OMEX file that was generated from the EnzymeML spreadsheet found in this dataset.

Notes:

text/plain

Other Study-Related Materials

Label:

EnzymeML_pH5,5.xlsm

Notes:

application/vnd.ms-excel.sheet.macroenabled.12

Other Study-Related Materials

Label:

EnzymeML_pH6,5.xlsm

Notes:

application/vnd.ms-excel.sheet.macroenabled.12

Other Study-Related Materials

Label:

EnzymeML_pH6.xlsm

Notes:

application/vnd.ms-excel.sheet.macroenabled.12

Other Study-Related Materials

Label:

EnzymeML_pH7,6.xlsm

Notes:

application/vnd.ms-excel.sheet.macroenabled.12

Other Study-Related Materials

Label:

EnzymeML_pH7.xlsm

Notes:

application/vnd.ms-excel.sheet.macroenabled.12

Other Study-Related Materials

Label:

EnzymeML_pH8.xlsm

Notes:

application/vnd.ms-excel.sheet.macroenabled.12