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Part 1: Document Description
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Citation |
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Title: |
Consensus sequences of arylsulfatases and phosphonate monoester hydrolases |
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Identification Number: |
doi:10.18419/darus-1838 |
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Distributor: |
DaRUS |
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Date of Distribution: |
2021-05-12 |
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Version: |
1 |
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Bibliographic Citation: |
Buchholz, Patrick C. F., 2021, "Consensus sequences of arylsulfatases and phosphonate monoester hydrolases", https://doi.org/10.18419/darus-1838, DaRUS, V1 |
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Citation |
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Title: |
Consensus sequences of arylsulfatases and phosphonate monoester hydrolases |
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Identification Number: |
doi:10.18419/darus-1838 |
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Authoring Entity: |
Buchholz, Patrick C. F. (Universität Stuttgart) |
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Distributor: |
DaRUS |
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Access Authority: |
Pleiss, Jürgen |
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Depositor: |
Buchholz, Patrick C. F. |
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Date of Deposit: |
2021-05-07 |
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Holdings Information: |
https://doi.org/10.18419/darus-1838 |
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Study Scope |
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Keywords: |
Medicine, Health and Life Sciences, amino acid sequence, protein sequence |
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Abstract: |
A multiple sequence alignment was constructed by Clustal Omega for the 95 protein sequences annotated as homologues of arylsulfatases and the 85 protein sequences annotated as homologues of phosphonate monoester hydrolases, respectively. The HMMER software suite was used to derive a profile hidden Markov model from a multiple sequence alignment via the command hmmbuild, and to derive a consensus sequence from a profile hidden Markov model via the command hmmemit. |
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Methodology and Processing |
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Sources Statement |
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Data Sources: |
van Loo B, Schober M, Valkov E, Heberlein M, Bornberg-Bauer E, Faber K, et al. Structural and Mechanistic Analysis of the Choline Sulfatase from Sinorhizobium melliloti: A Class I Sulfatase Specific for an Alkyl Sulfate Ester. J Mol Biol. 2018;430: 1004-1023. <a href="https://doi.org/10.1016/j.jmb.2018.02.010">doi:10.1016/j.jmb.2018.02.010</a> |
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van Loo B, Bayer CD, Fischer G, Jonas S, Valkov E, Mohamed MF, et al. Balancing Specificity and Promiscuity in Enzyme Evolution: Multidimensional Activity Transitions in the Alkaline Phosphatase Superfamily. J Am Chem Soc. 2019;141: 370-387. <a href="https://doi.org/10.1021/jacs.8b10290">doi:10.1021/jacs.8b10290</a> |
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Data Access |
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Other Study Description Materials |
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Related Studies |
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<a href="https://doi.org/10.18419/darus-1801">https://doi.org/10.18419/darus-1801</a> |
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Related Publications |
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Citation |
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Title: |
Patrick C. F. Buchholz, Bert van Loo, Bernard D. G. Eenink, Erich Bornberg-Bauer, Jürgen Pleiss: "Ancestral sequences of a large promiscuous enzyme family correspond to bridges in sequence space in a network representation" (submitted) |
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Bibliographic Citation: |
Patrick C. F. Buchholz, Bert van Loo, Bernard D. G. Eenink, Erich Bornberg-Bauer, Jürgen Pleiss: "Ancestral sequences of a large promiscuous enzyme family correspond to bridges in sequence space in a network representation" (submitted) |
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Label: |
AS-consensus.fasta |
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Text: |
FASTA file containing the consensus protein sequence of arylsulfatases based on the profile hidden Markov model in AS.hmm |
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Notes: |
application/octet-stream |
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Label: |
AS.aln |
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Text: |
Multiple sequence alignment in Clustal format for 95 homologues of arylsulfatases |
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Notes: |
application/octet-stream |
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Label: |
AS.hmm |
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Text: |
Profile hidden Markov model in HMMER3 format based on the multiple sequence alignment in AS.aln |
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Notes: |
application/octet-stream |
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Label: |
PMH-consensus.fasta |
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Text: |
FASTA file containing the consensus protein sequence of phosphonate monoester hydrolases based on the profile hidden Markov model in PMH.hmm |
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Notes: |
application/octet-stream |
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Label: |
PMH.aln |
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Text: |
Multiple sequence alignment in Clustal format for 85 homologues of phosphonate monoester hydrolases |
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Notes: |
application/octet-stream |
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Label: |
PMH.hmm |
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Text: |
Profile hidden Markov model in HMMER3 format based on the multiple sequence alignment in PMH.aln |
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Notes: |
application/octet-stream |